碳状颗粒物促进了抗生素耐药性基因的水平转移
Xuexia Peng1, Jiake Zhou1, Zishu Lan1
1Tianjin Institute of Environmental and Operational Medicine, Key Laboratory of Risk Assessment and Control for Environment & Food Safety, No. 1 Dali Road, Tianjin 300050, P. R. China. yangd8611@163.com.
Environmental science. Processes & impacts
|April 15, 2024
概括
碳状颗粒物 (CPM) 在空气中环境中显著增强了抗生素耐药性基因 (ARG) 的转移. 较小的CPM颗粒和较高的度增加了ARG转移,造成环境风险.
科学领域:
- 环境科学 环境科学
- 微生物学 微生物学
- 毒理学 毒理学 毒理学
背景情况:
- 抗生素耐药性基因 (ARG) 和抗生素耐药性细菌 (ARB) 是新兴的环境污染物.
- 空气中的颗粒物是ARG传播的潜在载体.
研究的目的:
- 调查碳状颗粒物 (CPM) 对ARG体外转移的影响.
- 为了确定CPM属性 (类型,度,颗粒大小) 和ARG传输频率之间的关系.
主要方法:
- 在体外实验中,将细菌暴露在各种类型的CPM (石墨烯,碳黑,纳米石墨粉,石墨粉) 中.
- 在不同的CPM度和颗粒大小中量化ARG传输频率.
- 分析细胞机制,包括反应性氧物种 (ROS) 生产,SOS反应和结合性转移基因的表达.
主要成果:
- 与对照组相比,CPM显著促进了ARG转移,其增加幅度从3.8到95.5倍.
- 随着CPM度的增加,ARG传输频率增加.
- 较小的CPM粒子大小与较高的结合转移频率相关.
- 通过增加ROS,激活SOS响应,并升调关键转移基因 (trbBp,trfAp,korA,kroB,trbA),CPM增强了ARG转移.
结论:
- 碳状颗粒物是空气中的ARG转移的重要促进者.
- 这些发现凸显了CPM与传播抗生素耐药性相关的环境风险.
- 这项研究强调了加强对空气中的污染物环境保护措施的必要性,这些污染物有助于抗生素耐药性的传播.
相关概念视频
02:18Types of Genetic Transfer Between Organisms
27.8K
Genetic transfer occurs when genetic information is passed from one organism to another. It occurs via two mechanisms: vertical gene transfer and horizontal gene transfer. Vertical gene transfer occurs when genetic information is transferred from one generation to the next, which happens much more frequently than horizontal gene transfer. Both sexual and asexual reproduction are forms of vertical gene transfer, where one or more organisms pass some or all of their genome onto their progeny.
27.8K
00:57Antibiotic Selection
53.3K
Overview
53.3K
00:46Genomic DNA in Prokaryotes
43.8K
The genome of most prokaryotic organisms consists of double-stranded DNA organized into one circular chromosome in a region of cytoplasm called the nucleoid. The chromosome is tightly wound, or supercoiled, for efficient storage. Prokaryotes also contain other circular pieces of DNA called plasmids. These plasmids are smaller than the chromosome and often carry genes that confer adaptive functions, such as antibiotic resistance.
Genomic Diversity in Bacteria
Although bacterial genomes are much...
Genomic Diversity in Bacteria
Although bacterial genomes are much...
43.8K
03:21Genome Size and the Evolution of New Genes
8.0K
While every living organism has a genome of some kind (be it RNA, or DNA), there is considerable variation in the sizes of these blueprints. One major factor that impacts genome size is whether the organism is prokaryotic or eukaryotic. In prokaryotes, the genome contains little to no non-coding sequence, such that genes are tightly clustered in groups or operons sequentially along the chromosome. Conversely, the genes in eukaryotes are punctuated by long stretches of non-coding sequence.
8.0K