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相关概念视频

Gastrulation01:56

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Gastrulation establishes the three primary tissues of an embryo: the ectoderm, mesoderm, and endoderm. This developmental process relies on a series of intricate cellular movements, which in humans transforms a flat, “bilaminar disc” composed of two cell sheets into a three-tiered structure. In the resulting embryo, the endoderm serves as the bottom layer, and stacked directly above it is the intermediate mesoderm, and then the uppermost ectoderm. Respectively, these tissue strata...
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相关实验视频

Updated: Jun 28, 2025

Isolation and Time-Lapse Imaging of Primary Mouse Embryonic Palatal Mesenchyme Cells to Analyze Collective Movement Attributes
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在小鼠中发展口味的时空空间进化.

B Wang1, Z Zhang2, J Zhao1

  • 1Center for Cleft Lip and Palate Treatment, Plastic Surgery Hospital, Chinese Academy of Medical Sciences and Peking Union Medical College, Beijing, China.

Journal of dental research
|April 15, 2024
PubMed
概括

这项研究使用单细胞RNA测序和空间奥米学绘制了 palatal 发育的地图,揭示了不同的细胞作用和信号通路,这对于 palatal 形成和潜在的出生缺陷洞察至关重要.

关键词:
在口腔裂口口腔.宫殿的宫殿是一个宫殿.产前发育 产前发育单细胞转录组分析空间转录组分析转录组的个人资料.

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Last Updated: Jun 28, 2025

Isolation and Time-Lapse Imaging of Primary Mouse Embryonic Palatal Mesenchyme Cells to Analyze Collective Movement Attributes
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科学领域:

  • 发育生物学 发展生物学
  • 基因组学就是基因组学.
  • 细胞生物学 细胞生物学

背景情况:

  • 口腔形成是复杂的,基础机制不太了解.
  • 识别关键细胞种群及其相互作用对于理解发育至关重要.

研究的目的:

  • 在发育过程中创建 palatal 细胞的时空转录地图.
  • 识别不同的细胞子集及其空间分布.
  • 分析涉及口腔形成的细胞间信号网络.

主要方法:

  • 单细胞RNA测序 (scRNA-seq) 在胚胎日 12.5,14.5 和 16.5.5 的小鼠口腔.
  • 空间增强分辨率的奥米克测序 (立体-seq) 来绘制细胞分布图.
  • 将scRNA-seq和立体seq数据与CellTrek集成,用于细胞注释和通信分析.

主要成果:

  • 在3个领域中识别和空间绘制了不同的介质细胞和上皮细胞群.
  • 中细胞亚种群与牙形成,骨化和重塑有关.
  • 鼻上皮表现出更强的免疫反应;特定信号 (Tgfβ3,Pthlh) 在中线部被确定.

结论:

  • 提供了高分辨率的口腔发育的转录基因地图.
  • 在发育的 palates 中阐明空间组织和细胞间通信.
  • 提供了对口腔生物学和口腔裂的潜在病理生理学的机制性见解.