通过植物类同素-6-基酶生产瓜尼丁
Dietmar Funck1, Malte Sinn1, Giuseppe Forlani2
1Department of Chemistry, University of Konstanz, Konstanz, Germany.
eLife
|April 15, 2024
概括
植物和藻类中的新酶从homoarginine中产生guanidine. 这一发现揭示了光合作用真核生物中瓜尼丁生产的以前被低估的途径,影响了循环.
科学领域:
- 生物化学 生物化学
- 植物科学 植物科学
- 代谢学 代谢学 代谢学
背景情况:
- 瓜尼丁的代谢和生物功能在很大程度上被忽视了.
- 已知的瓜尼丁生产途径仅限于少数细菌和真菌.
- 瓜尼丁感应的核糖开关和细菌中的降解途径表明更广泛的自然发生.
研究的目的:
- 在光合作用真核生物中确定负责瓜尼丁生产的新型酶.
- 描述植物和藻类衍生的二氧化原酶的酶活性和基质特异性.
- 调查Arabidopsis.在瓜尼丁生产的生理相关性和调节.
主要方法:
- 使用来自植物和藻类的Fe2+和2-oxoglutarate依赖的二氧化基酶 (2-ODD-C23) 的生物化学分析.
- 对同类氨酸氧化和随后的自发衰变产物的分析.
- 用不同的基质 (homoarginine, arginine) 进行酶活性测定.
- 在野生型,突变型和过度表达的阿拉比多普西斯植物中测量guanidine含量.
- 用甲基雅斯蒙酸盐对植物进行处理,以诱导酶表达.
主要成果:
- 在植物和藻类中,一种保存的2-ODD-C23酶类型催化了homoarginine氧化.
- 该产品,6-氧荷马尔金因,自发产生瓜尼丁和2-氨基酸-6-半化物.
- 阿拉比多普西斯Din11表现出独特的基质接受 (氨酸) 并不产生乙烯.
- 在阿拉比多普西斯的关尼丁含量在同质氨酸养或甲基亚斯莫纳酸治疗后增加.
- 2-ODD-C23的三重突变显示了瓜尼丁含量降低,而过度表达线则显示了水平的增加.
结论:
- 在光合作用真核生物中存在广泛的瓜尼丁生成酶 (2-ODD-C23).
- 这代表了生物地化学循环的重要,以前被低估的分支.
- 需要进一步的研究来阐明植物和藻类天然瓜尼丁生产的精确生物功能.
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