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脱氧尼瓦伦醇诱导的猪小肠上皮细胞的炎症损伤与葡萄糖运输的减少有关
Xiaopeng Tang1, Yan Zeng2, Kangning Xiong1
1State Engineering Technology Institute for Karst Desertfication Control, School of Karst Science, Guizhou Normal University, Guiyang 5500025, China.
Journal of animal science
|April 15, 2024
概括
脱氧尼瓦伦醇 (DON) 引起炎症损伤,并损害了猪肠细胞中葡萄糖的吸收. 这种损伤与像SGLT1.1这样的葡萄糖载体的表达减少有关.
科学领域:
- 胃肠病学 胃肠病学
- 毒理学 毒理学 毒理学
- 细胞生物学 细胞生物学
背景情况:
- 脱氧尼瓦伦醇 (DON) 是一种可以引起胃肠道问题的真菌毒素.
- 了解DON对肠道细胞的影响对于动物健康和食品安全至关重要.
研究的目的:
- 调查DON对猪肠细胞炎症损伤的影响 (IPEC-J2).
- 研究DON对葡萄糖载体 (SGLT1,GLU2) 和葡萄糖吸收的影响.
- 探索DON暴露细胞中炎症和葡萄糖运输之间的关系.
主要方法:
- IPEC-J2细胞暴露于DON (1.0微克/毫升24小时).
- 评估了细胞活力,细胞亡和炎症性细胞因子 (IL-1β,IL-6,TNF-α).
- 测量了葡萄糖吸收标记 (葡萄糖含量,AKP,Na+/K+-ATPase活性) 和输送体表达 (AMPK-α1,SGLT1,GLU2).
主要成果:
- 暴露于DON显著增加了细胞损伤,细胞亡和促炎性细胞因子的释放.
- DON显著降低了葡萄糖吸收能力,由降低的葡萄糖含量,AKP,Na+/K+-ATPase活性,AMPK-α1和SGLT1表达体现出来.
- 在葡萄糖吸收能力和炎症标志物之间发现了负相关性.
结论:
- 在猪的肠道细胞中,DON会诱导炎症性损伤.
- 通过降低关键输送物和相关蛋白质的调节,DON会损害葡萄糖的吸收.
- DON诱导的肠道炎症与葡萄糖吸收能力的降低有关.
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