1 免疫功能低下和感染动物的管理
H Mossmann1, W Nicklas2, Hj Hedrich3
1Deutsches Krebsforschungszentrum, Heidelberg, Germany.
Methods in microbiology
|April 15, 2024
概括
实验室动物的有效管理和监测对于确保微生物质量至关重要. 包括血清学和病理学在内的全面测试对于免疫功能低下的动物和感染研究至关重要,以检测潜在的污染物.
科学领域:
- 实验室动物科学 动物科学
- 微生物学 微生物学
- 生殖生物学 生殖生物学
背景情况:
- 实验动物的微生物学质量取决于殖民地管理.
- 监测评估管理实践的适当性.
- 对于免疫力低下的动物和感染实验来说,全面的微生物监测是必不可少的.
研究的目的:
- 讨论免疫受损和感染动物的管理.
- 概述实验室动物的微生物监测策略.
- 详细说明胚胎冷保存和复苏方案,以建立繁殖核.
主要方法:
- 尸体解剖,血清学血液采样和对寄生虫的显微镜检查.
- 器官采样用于细菌学,病理学和病毒学.
- 植入前胚胎的冷保存 (推八细胞阶段) 和恢复率的评估.
主要成果:
- 来自动物的生物材料具有很高的污染风险,特别是通过瘤,病毒或寄生虫.
- 植入前胚胎 (老鼠和老鼠) 可以在冷解后成功地恢复生命.
- 建议将八细胞胚胎长期存储在两个细胞阶段.
结论:
- 严格的殖民地管理和全面的监测是保持实验室动物质量的关键.
- 标准化胚胎冷保存协议确保了繁殖核的建立.
- 建议每批至少10个胚胎,以获得50%的机会获得肥沃的繁殖对,假设再生率为20%.
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