Jove
Visualize
联系我们
JoVE
x logofacebook logolinkedin logoyoutube logo
关于 JoVE
概览领导团队博客JoVE 帮助中心
作者
出版流程编辑委员会范围与政策同行评审常见问题投稿
图书馆员
用户评价订阅访问资源图书馆顾问委员会常见问题
研究
JoVE JournalMethods CollectionsJoVE Encyclopedia of Experiments存档
教育
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab Manual教师资源中心教师网站
使用条款与条件
隐私政策
政策

相关概念视频

Proteomics01:33

Proteomics

7.3K
A proteome is the entire set of proteins that a cell type produces. We can study proteomes using the knowledge of genomes because genes code for mRNAs, and the mRNAs encode proteins. Although mRNA analysis is a step in the right direction, not all mRNAs are translated into proteins.
Proteomics is the study of proteomes' function. It involves the large-scale systematic study of the proteome to denote the protein complement expressed by a genome. Scientist Mark Wilkins coined the term...
7.3K

您也可能阅读

相关文章

通过共同作者、期刊和引用图与本文相关的文章。

排序
Same author

Therapy and biomarker dependent progression-free survival in infant sonic hedgehog medulloblastoma: a multi-national retrospective cohort study.

EClinicalMedicine·2026
Same author

Genetic discrimination in workplace, insurance industry and legal system: A review.

Legal medicine (Tokyo, Japan)·2026
Same author

Lewy bodies are surrounded by granulovacuolar degeneration bodies in dementia with Lewy bodies.

Brain pathology (Zurich, Switzerland)·2026
Same author

The seminal proteome of a monandrous fly, Drosophila subobscura.

Proceedings. Biological sciences·2026
Same author

Integrated biomarker and treatment correlates of prognosis in infant non-WNT/non-SHH medulloblastoma: a multinational retrospective cohort study.

The Lancet. Child & adolescent health·2026
Same author

Simulating Compassion: Enhancing Graduate Nurses' Preparedness for End-of-Life and Palliative Care.

Nursing education perspectives·2026
Same journal

Identification of Age-Associated Circulating Proteins and Lipids in 3800 Comorbidity-Enriched Older Adults from Japan-Based Cohorts Using Olink Assays and MRM Mass Spectrometry.

Journal of proteome research·2026
Same journal

Molecular Solution to the Paradox of Ancient Brain Preservation.

Journal of proteome research·2026
Same journal

From Method-Defined Signals to Reference Measurement Procedures: Two Decades of Mass Spectrometry-Based ProGRP Quantification.

Journal of proteome research·2026
Same journal

Proteomic Profiling of Extracellular Vesicle-Enriched Plasma Using Mag-Net for Biomarker Discovery in Pancreatic Ductal Adenocarcinoma.

Journal of proteome research·2026
Same journal

Computationally Efficient Bayesian Estimation of Graphical Networks for Omics Data.

Journal of proteome research·2026
Same journal

Hierarchy of MS-Based Evidence.

Journal of proteome research·2026
查看所有相关文章

相关实验视频

Updated: Jun 28, 2025

Optimized Bone Sampling Protocols for the Retrieval of Ancient DNA from Archaeological Remains
06:18

Optimized Bone Sampling Protocols for the Retrieval of Ancient DNA from Archaeological Remains

Published on: November 30, 2021

3.8K

骨蛋白学方法优化用于法医调查

Luke Gent1, Maria Elena Chiappetta1,2, Stuart Hesketh3

  • 1School of Law and Policing, Research Centre for Field Archaeology and Forensic Taphonomy, University of Central Lancashire, Preston PR1 2HE, United Kingdom.

Journal of proteome research
|April 15, 2024
PubMed
概括
此摘要是机器生成的。

这项研究优化了用于法医科学的骨蛋白质组分析. S-Trap方法和数据独立获取 (DIA) 改善了骨遗骸中的蛋白质识别和可重复性.

关键词:
收购模式 收购模式骨蛋白质组学 骨蛋白质组学 骨蛋白质组学法医科学 法医科学 法医科学质谱测量质谱测量质谱测量质谱测量质量测量质谱测量质量测量质量测量质量测量质量测量质量测量质量测量质量测量质量测量质量测量质量测量质量测量质量测量质量测量质量测量质量测量质量测量质量测量质量测量质量测量蛋白质提取 蛋白质提取

更多相关视频

Scanning Skeletal Remains for Bone Mineral Density in Forensic Contexts
07:56

Scanning Skeletal Remains for Bone Mineral Density in Forensic Contexts

Published on: January 29, 2018

17.5K
Skeletal Muscle Gender Dimorphism from Proteomics
09:29

Skeletal Muscle Gender Dimorphism from Proteomics

Published on: December 14, 2011

12.5K

相关实验视频

Last Updated: Jun 28, 2025

Optimized Bone Sampling Protocols for the Retrieval of Ancient DNA from Archaeological Remains
06:18

Optimized Bone Sampling Protocols for the Retrieval of Ancient DNA from Archaeological Remains

Published on: November 30, 2021

3.8K
Scanning Skeletal Remains for Bone Mineral Density in Forensic Contexts
07:56

Scanning Skeletal Remains for Bone Mineral Density in Forensic Contexts

Published on: January 29, 2018

17.5K
Skeletal Muscle Gender Dimorphism from Proteomics
09:29

Skeletal Muscle Gender Dimorphism from Proteomics

Published on: December 14, 2011

12.5K

科学领域:

  • 法医蛋白质组学 法医蛋白质组学
  • 生物分子分析.
  • 质谱测量质量谱测量

背景情况:

  • 骨遗骸的蛋白质组分析有助于法医调查.
  • 实现最大限度的蛋白质回收和最大限度地减少实验室错误对于法医案例工作至关重要.
  • 为了获得可靠的结果,需要对蛋白质组工作流程进行标准化.

研究的目的:

  • 为了比较不同的骨蛋白提取和分析工作流程,用于法医应用.
  • 评估新型S-Trap技术和数据采集方法 (DDA与DIA) 对蛋白质组结果的影响.
  • 提高法医骨样本中蛋白质组分析的可复制性和灵敏度.

主要方法:

  • 在StageTip,S-Trap与溶解溶液1和S-Trap与溶解溶液2工作流程的比较.
  • 分析了30个人类皮质骨样本,使用液态色谱与联质谱 (LC-MS/MS).
  • 对数据依赖获取 (DDA) 与数据独立获取 (DIA) 进行蛋白质识别的评估.

主要成果:

  • 与StageTip相比,S-Trap工作流产生了更高的蛋白质复原和减少诱导的除化.
  • 数据独立获取 (DIA) 显示出更高的灵敏度和更广泛的蛋白质识别,特别是低丰度蛋白质.
  • 开源软件在DDA和DIA模式中增强了蛋白质识别.

结论:

  • S-Trap协议非常适合用于法医骨蛋白质组分析.
  • 将S-Trap与DIA相结合,为法医应用提供了更好的蛋白质组结果和可重复性.
  • 这种优化的工作流有助于在法医科学中标准化骨蛋白质组分析.