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环境参数对Cupriavidus necator单细胞蛋白质生产和组成的影响

Siwar Ismail¹, Géraldine Giacinti², Christine Delagado Raynaud²

¹TBI, Université de Toulouse, CNRS, INRAE, INSA, Toulouse, France.

Journal of biotechnology

|April 15, 2024

概括

来自Cupriavidus necator的单细胞蛋白 (SCP) 是一个有希望的替代蛋白质来源. 最佳生产发生在30°C和pH7下,但极端条件降低蛋白质产量并增加聚3-基酸盐 (PHB) 积累.

科学领域:

微生物学微生物学
生物技术是生物技术.
营养科学营养科学

背景情况:

全球人口增长加剧了营养不良和粮食不安全,特别是蛋白质缺乏.
单细胞蛋白 (SCP) 是一种非常规的蛋白质来源,可以应对这些营养挑战.
库普里亚维杜斯 (Cupriavidus necator) 被探索为SCP生产的微生物候选者.

研究的目的:

调查环境条件 (温度和pH值) 对Cupriavidus necator的SCP生产和组成的影响.
在不同的条件下分析细菌生长,蛋白质含量,核酸,氨基酸和元素配方.
通过流细胞计,评估细胞形态,膜透性和聚3-基酸盐 (PHB) 积累.

主要方法:

使用Cupriavidus necator的批量生物反应器培养的单因子方法.
控制温度 (例如30°C,40°C) 和pH (例如pH5,pH7) 的变化.
通过流细胞计量分析SCP组成 (蛋白质,核酸,氨基酸,元素配方) 和细胞特征.

主要成果:

在30°C和pH7.0下,Cupriavidus necator表现出高蛋白质产量 (69%的干重).
温度和pH值独立地影响了SCP的产生和组成.
低于最佳条件 (40°C或pH5) 导致生长减缓,细胞延长,蛋白质/核酸含量降低,PHB积累增加 (干重高达44%).

结论:

环境因素显著影响Cupriavidus necator中的SCP产量和质量.
最佳条件对于最大限度地提高蛋白质含量和最大限度地减少PHB积累至关重要.
这项研究报告了Cupriavidus necator在营养不限条件下的PHB积累,突出了其代谢灵活性.

关键词:

氨基酸是氨基酸的组成部分.细菌细菌是一种细菌.批量文化是一种批量文化.微生物蛋白质是一种微生物蛋白质.核酸是核酸中的一种.聚氨酸多基甲基酸温度温度是指温度.它们的pH值为pH.

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