尿中血小板糖代谢物的参考间隔建立和相关性研究:揭示血小板激活的新可能性
Yunfeng Cao1, Furong Zhao2, Shuang Wang3
1Shanghai-MOST Key Laboratory of Health and Disease Genomics, NHC Key Lab of Reproduction Regulation, Shanghai institute for Biomedical and Pharmaceutical Technologies, Shanghai, China.
概括
这项研究开发了一种LC-MS/MS方法,用于同时测量尿液中的两种血栓素代谢物,11-脱-血栓素B2 (11dhTxB2) 和11-脱-2,3-丁血栓素B2 (11dh23dinorTxB2). 建立参考间隔并发现它们的相关性为心血管疾病提供了新的诊断可能性.
科学领域:
- 生物化学 生物化学
- 临床化学 临床化学
- 分析化学 分析化学
背景情况:
- 血小板激活的间接标记物是血小板的代谢物,例如11-脱-血小板B2 (11dhTxB2) 和11-脱-2,3-丁血小板B2 (11dh23dinorTxB2).
- 这些稳定的尿路代谢物与疾病进展和药物使用有关,但临床研究通常仅集中在11dhTxB2.2.上.
- 为了进行全面的临床评估,需要同时确定两种代谢物.
研究的目的:
- 开发和验证一种液体染色学-并联质谱法 (LC-MS/MS) 方法,用于同时量化尿中的11dhTxB2和11dh23dinorTxB2.
- 在健康成年人中为这些代谢物建立参考间隔 (RI).
- 在健康个体和冠状动脉样硬化 (CA) 患者中研究尿路11dhTxB2和11dh23dinorTxB2之间的相关性.
主要方法:
- 尿液样本使用液体-液体提取方法进行处理.
- 用经过验证的LC-MS/MS方法对11dhTxB2和11dh23dinorTxB2进行了量化.
- 在333名健康成年人中建立了参考间隔,并在25名CA患者中进行了验证.
主要成果:
- LC-MS/MS方法证明了广的定量范围 (0.1-10μmol/L) 具有良好的精度 (5.2%-11%) 和准确性 (89.3%-106.5%).
- 第95个百分点的参考间隔为11dhTxB2的1220 pg/mg Cr和11dh23dinorTxB2.2的908 pg/mg Cr.
- 在健康成年人 (r=0.67) 和CA患者 (r=0.77) 中观察到11dhTxB2和11dh23dinorTxB2之间存在显著的正相关性.
结论:
- 建立的参考间隔为评估血小板激活和药物效应提供了有价值的基准.
- 尿路11dhTxB2和11dh23dinorTxB2之间存在相关性的新发现为诊断和预测心血管疾病开辟了新的途径.
- 这种同时进行LC-MS/MS的方法适用于例行临床查.
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