多模块化代谢工程和排放工程,用于在重组Saccharomyces cerevisiae中增强甘生产
Guangxi Huang1,2, Jiarong Li1,2, Jingyuan Lin1,2
1C entre for Viticulture and Enology, College of Food Science and Nutritional Engineering, China Agricultural University, Beijing 100083, China.
Journal of industrial microbiology & biotechnology
|April 15, 2024
概括
研究人员对酵母进行了改造,以高效地产生价值的抗氧化剂 - - 烯. 这种代谢工程方法显著提高了烯的产量,为工业应用提供了可扩展的解决方案.
科学领域:
- 代谢工程是代谢工程.
- 合成生物学 合成生物学
- 生物技术是生物技术.
背景情况:
- 百合素是一种强大的抗氧化剂,在食品和医药中具有应用.
- 通过微生物发酵进行工业规模的烯生产面临重大挑战.
- 有效的生物合成需要优化代谢途径和强大的生产系统.
研究的目的:
- 在Saccharomyces cerevisiae中开发一个高效和平衡的烯生物合成系统.
- 通过综合代谢工程策略来提高烯产量.
- 建立一个可扩展的平台,用于工业用烯生产.
主要方法:
- 模块化工程的甲酸盐路径,乙-CoA供应,和烯合成模块.
- 使用乙酸作为碳源和乙酸可抑制的促进剂来重定向代谢流量.
- 优化NADPH供应,并通过使用ABC输送器增强烯流量.
主要成果:
- 通过途径模块化实现了烯产量的3.1倍增加.
- 通过将新陈代谢流量从厄戈斯特路径转向,莱科生产进一步增加了42.3%.
- 细胞外利科增加了12.7倍,达到343.7mg/L的总产量 (比初始菌株高4.3倍).
结论:
- 综合的多模块代谢工程与排放工程相结合,有效地提高了烯的生产.
- 开发的战略为工业烯制造提供了强大的和可扩展的方法.
- 这种方法适用于酵母中其他有价值的异oprenoid 化合物的过度生产.
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