由CTBP1-AS2/miR-335-5p轴调节的CCL2促进了血管瘤的进展和血管生成
Ruixuan Li1, Ying Liu2, Jianfeng Liu2
1Department of Cardiology, the Third Xiangya Hospital, Central South University, Changsha, PR China.
Immunopharmacology and immunotoxicology
|April 15, 2024
概括
这项研究表明,CTBP1-AS2/miR-335-5p/CCL2通路促进了血管瘤 (HA) 的生长和血管生成. 针对这一轴为HA治疗提供了一个潜在的治疗策略.
科学领域:
- 血管生物学 血管生物学
- 分子瘤学分子瘤学
- 生物化学 生物化学
背景情况:
- 血瘤 (HA) 是一种与痕相关的良性血管瘤.
- C-C 动机化学因子连接体2 (CCL2) 参与HA生长和血管生成.
- 在HA中CCL2的调节机制尚不清楚.
研究的目的:
- 阐明调节血瘤中CCL2表达的分子机制.
- 研究长非编码RNACTBP1-AS2和microRNAmiR-335-5p在HA病变发生中的作用.
主要方法:
- 定量实时PCR (RT-qPCR) 用于测量基因表达.
- 在人类HA内皮细胞 (HemECs) 中进行细胞测试 (增殖,迁移,入侵,血管生成).
- 生物信息学,RNA pull-down,和 luciferase 记者测试来确定分子相互作用.
主要成果:
- 在HemEC中,CCL2表达显著上调,促进了HA细胞的增殖,迁移,入侵和血管生成.
- CCL2是miR-335-5p的直接目标.
- CTBP1-AS2通过菌miR-335-5p作为竞争的内源RNA (ceRNA) 起作用,导致CCL2水平增加.
结论:
- CTBP1-AS2/miR-335-5p/CCL2轴在血管瘤进展中发挥着至关重要的作用.
- 针对这一轴,为血液瘤治疗提供了潜在的治疗途径.
更多相关视频
08:50In Vitro Cultivation Techniques for Modeling Liver Organogenesis, Building Assembloids, and Designing Synthetic Tissues using Human Cell Lines
Published on: April 18, 2025
390
07:26Quantitative PCR-based Assay to Measure Sonic Hedgehog Signaling in Cellular Model of Ciliogenesis
Published on: January 31, 2025
511
相关概念视频
01:24Regulation of Angiogenesis and Blood Supply
2.6K
Rapidly dividing tumors, embryos, and wounded tissues require more oxygen than usual, lowering the oxygen concentration in the blood. At low oxygen or hypoxic conditions, an oxygen-sensitive transcription factor called the hypoxia-inducible factor 1 or HIF1 is activated. HIF1 is a dimeric protein of alpha (ɑ) and beta (β) subunits. Under optimal oxygen conditions, HIF1β is present in the nucleus while HIF1ɑ remains in the cytosol. HIF1ɑ is hydroxylated by prolyl...
2.6K
02:33Hedgehog Signaling Pathway
7.3K
The Hedgehog gene (Hh) was first discovered due to its control of the growth of disorganized, hair-like bristles phenotype in Drosophila, much like hedgehog spines. Hh plays a crucial role in the development of organs and the maintenance of homeostasis in both invertebrates and vertebrates. However, while Drosophila has only one Hh protein, mammals have multiple functional Hedgehog proteins - Sonic (Shh), Desert (Dhh), and Indian Hedgehog (Ihh). All of these homologous proteins have adapted to...
7.3K
01:10Mechanism of Angiogenesis
5.4K
Blood vessel formation starts early during embryonic development, around day 7. In the extraembryonic yolk sac, mesodermal precursor cells called hemangioblast proliferate and differentiate into angioblast. Angioblasts express vascular endothelial growth factor receptor 2 or VEGFR2, which binds VEGF-A, a proangiogenic factor, guiding blood vessel formation. VEGF signaling promotes angioblasts to form a blood island in the developing embryo. Angioblasts further differentiate, giving rise to...
5.4K
01:01Regulation of Hematopoietic Stem Cells
3.2K
All blood and immune cells are produced from the multipotent hematopoietic stem cells (HSCs) by the process of hematopoiesis. However, they all have a limited life span. In addition, many are depleted in immune surveillance or combatting an injury or infection. This makes blood one of the most regenerative tissues. Hematopoiesis helps replenish these blood and immune cells, restoring the body's normal functioning. However, overproduction of blood and immune cells can make them cancerous or...
3.2K
02:23Abnormal Proliferation
4.5K
Under normal conditions, most adult cells remain in a non-proliferative state unless stimulated by internal or external factors to replace lost cells. Abnormal cell proliferation is a condition in which the cell's growth exceeds and is uncoordinated with normal cells. In such situations, cell division persists in the same excessive manner even after cessation of the stimuli, leading to persistent tumors. The tumor arises from the damaged cells that replicate to pass the damage to the...
4.5K
03:03mTOR Signaling and Cancer Progression
3.8K
The mammalian target of rapamycin or mTOR protein was discovered in 1994 due to its direct interaction with rapamycin. The protein gets its name from a yeast homolog called TOR. The mTOR protein complex in mammalian cells plays a major role in balancing anabolic processes such as the synthesis of proteins, lipids, and nucleotides and catabolic processes, such as autophagy in response to environmental cues, such as availability of nutrients and growth factors.
The mTOR pathway or the...
The mTOR pathway or the...
3.8K