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通过单细胞分析对人类雄原体的纵向分析揭示了胚胎早期发育中的父基因表达动态
X Vendrell1, P de Castro2, L Escrich3
1Reproductive Genetics Department, Sistemas Genómicos-Synlab, Valencia, Spain.
Human reproduction (Oxford, England)
|April 15, 2024
概括
人类雄性体胚胎在早期发育过程中显示出父亲基因组激活. 基因表达在第四个细胞周期达到顶峰,揭示了独特的父亲对胚胎基因组激活的贡献.
科学领域:
- 生殖生物学 生殖生物学
- 基因组学就是基因组学.
- 发展生物学 发展生物学
背景情况:
- 胚胎发育依赖于母体和父体的基因组.
- 使用纯平类雄原体对父亲基因组贡献的研究是有限的.
- 了解父亲基因组的作用对于早期胚胎发育至关重要.
研究的目的:
- 在单细胞水平上研究人类雄性体胚胎的转录组变异.
- 分析父亲基因组在前四个细胞周期中的贡献.
- 描述父基因组在胚胎胚胎基因组激活中的作用.
主要方法:
- 单细胞转录组测序38个androgenotes从10个体外生产的androgenote生物结构.
- 使用g:Profiler,GSEA,Cytoscape和Reactome的生物信息分析.
- 使用DESeq2.2进行差异基因表达分析.
主要成果:
- 在第三和第四个细胞周期中发生了一波由父驱动的转录基因激活.
- 与双亲胚胎相比,在雄原体中显著上调LEUTX,PRAMEF1,DUXA,RFPL4A,TRIM43和ZNF675等基因.
- 基因表达在第四个细胞周期达到顶峰,在一些androgenotes中具有明显的转录行为.
结论:
- 人类父亲的单双基因组在转录基因层面上显著影响了早期胚胎发育.
- 安德罗基诺特生物构造可以作为研究父亲基因组作用的有价值模型.
- 这些发现为了解父亲基因组在早期发育中的遗传贡献奠定了基础.
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