一个被氧调节的,异构的NADH:cinnamate降解酶在Vibrio ruber
Yulia V Bertsova1, Marina V Serebryakova2, Victor A Anashkin3
1Belozersky Institute of Physico-Chemical Biology, Lomonosov Moscow State University, Moscow, 119234, Russia. bertsova@belozersky.msu.ru.
Biochemistry. Biokhimiia
|April 15, 2024
概括
研究人员发现了一种新的酶,Crd,可以减少Vibrio ruber中的肉酸化合物. 这种依赖NADH的肉酸减少酶可以保护细菌免受有毒 (氧) 肉酸化合物的影响.
科学领域:
- 微生物学和生物化学
- 酶的功能和调节
背景情况:
- 在无氧微生物中,作用于α,β不和炭酸的减少酶的基因是常见的,但它们的基质特异性往往未得到证实.
- 了解这些还原酶对于阐明微生物新陈代谢和排毒途径至关重要.
研究的目的:
- 从Vibrio ruber中鉴定出一种新型的异体缩酶 (Crd),重点关注其基质特异性,调节和潜在的保护作用.
- 用同表达子单位研究Crd的酶活性和辅因子需求.
主要方法:
- 来自Vibrio ruber的两个子单元 (SJN56019和SJN56021) 与Escherichia coli中的Vibrio cholerae黄转移酶的同时表达.
- 纯化和表征异构蛋白 (Crd) 并评估其减少酶活性对各种α,β不和碳素酸的评估,使用NADH或甲基生物作为电子捐赠体.
- 酶动力学的分析,包括时间滞后研究,以及对V. ruber细胞中肉酸盐诱导酶的研究.
主要成果:
- 纯化的Crd酶很容易减少肉酸,p-coumarate,咖啡酸和酸盐,但对烯酸盐表现出中度活性,对尿酸酸和烟酸盐不活跃.
- 酶活性与NADH呈现时间滞后,表明有氧还原调节,而氧化酶是不活跃的,阻止了活性氧物种的产生.
- 在有氧和无氧生长条件下,肉酸化合物诱导了V. ruber中Crd的合成.
结论:
- 在Vibrio ruber中,Crd被确定为一种受调节的,依赖NADH的肉酸盐还原酶.
- 该酶可能通过排毒 (氧) 肉酸盐化合物来起保护作用,防止细胞中毒.
相关概念视频
Role of Reduced Coenzymes NADH and FADH₂
11.5K
The energy released from the breakdown of the chemical bonds within nutrients can be stored either through the reduction of electron carriers or in the bonds of adenosine triphosphate (ATP). In living systems, a small class of compounds functions as mobile electron carriers, molecules that bind to and shuttle high-energy electrons between compounds in pathways. The principal electron carriers that will be considered originate from the B vitamin group and are derivatives of nucleotides; they are...
11.5K
Electron Transport Chain: Complex I and II
13.1K
The mitochondrial electron transport chain (ETC) is the main energy generation system in the eukaryotic cells. However, mitochondria also produce cytotoxic reactive oxygen species (ROS) due to the large electron flow during oxidative phosphorylation. While Complex I is one of the primary sources of superoxide radicals, ROS production by Complex II is uncommon and may only be observed in cancer cells with mutated complexes.
ROS generation is regulated and maintained at moderate levels necessary...
ROS generation is regulated and maintained at moderate levels necessary...
13.1K
Electron Transport Chain: Complex III and IV
7.4K
During the electron transport chain, electrons from NADH and FADH2 are first transferred to complexes I and II, respectively. These two complexes then transfer the electrons to ubiquinol, which carries them further to complex III. Complex III passes the electrons across the intermembrane space to Cyt c, which carries them further to complex IV. Complex IV donates electrons to oxygen and reduces it to water. As electrons pass through complexes I, III, and IV, the energy released aids the pumping...
7.4K
The Photochemical Reaction Center
4.1K
Reaction centers are pigment-protein complexes that initiate energy conversion from photons to chemical entities. Therefore, photochemical reaction center is a more appropriate term that describes these complexes. The Nobel laureates Robert Emerson and William Arnold provided the first experimental evidence of photochemical reaction centers by demonstrating the participation of nearly 2,500 chlorophyll molecules for the release of just one molecule of oxygen. Despite thousands of photosynthetic...
4.1K
The Supercomplexes in the Crista Membrane
2.5K
The mitochondrial cristae membrane is the primary site for the oxidative phosphorylation (OXPHOS) process of energy conversion mediated through respiratory complexes I to V. These complexes have been widely studied for decades, and it has been proven that they form supramolecular structures called respiratory supercomplexes (SC). These higher-order complexes may be crucial in maintaining the biochemical structure and improving the physiological activity of the individual complexes while...
2.5K
Redox Equilibria: Overview
564
A reduction-oxidation reaction is commonly called a redox reaction. In a redox reaction, electrons are transferred from one species to another rather than being shared between or among atoms. The reducing agent or reductant is the species that loses electrons and gets oxidized in the process. The species that gains electrons and gets reduced in the process is the oxidizing agent or oxidant. Redox reactions are represented as two separate equations called half-reactions, where one equation...
564


![Protein Film Infrared Electrochemistry Demonstrated for Study of H2 Oxidation by a [NiFe] Hydrogenase](/_next/image?url=https%3A%2F%2Fcloudfront.jove.com%2FCDNSource%2Fteasers%2F55858.jpg&w=3840&q=50)