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类似Sm的蛋白质Rof通过结合部位阻塞和形状绝缘来抑制转录终结因子 ρ

Nelly Said¹, Mark Finazzo², Tarek Hilal^1,3

¹Laboratory of Structural Biochemistry, Institute of Chemistry and Biochemistry, Freie Universität Berlin, Takustr. 6, D-14195 Berlin, Germany.

Nature communications

|April 15, 2024

概括

蛋白质Rof通过与转录终结因子Rho结合并阻止其环状结构的形成来抑制转录终结因子Rho. 这种互动对于Rof来说至关重要.

科学领域:

分子生物学分子生物学
结构生物学结构生物学
遗传学是一个遗传学.

背景情况:

转录终结因子Rho是基因表达的关键调节者.
类似Sm的蛋白质Rof抑制Rho依赖终止,但其机制尚不清楚.
了解Rof的功能对于控制细菌基因表达至关重要.

研究的目的:

阐明Rof抑制Rho的分子机制.
为了确定Rho-Rof相互作用的结构基础.
研究Rof与Rho结合的功能后果.

主要方法:

电子显微镜 (cryo-EM) 用于确定Rho-Rof和Rho-RNA复合物的结构.
结构引导的突变发生,以确定关键的相互作用残留物.
在体外生化测试以评估Rho活性和Rof抑制.
生物信息分析评估Rof保护和基因组背景.

主要成果:

冷-EM结构显示Rof与Rho在原质体接口上结合,诱导着形状变化.
Rof结合可以防止Rho环的闭合,并封闭必要的RNA结合部位.
结构引导的突变发生证实了Rho-Rof接口对Rof的抑制功能至关重要.
在Pseudomonadota中保留了Rof,其独特的基因组背景表明了多种调节.

结论:

Rof通过物理阻断其构造变化和RNA结合来抑制Rho-依赖的转录终止.
已识别的Rho-Rof接口对于Rof的生物活动至关重要.
在特定的细菌系中,Rof可能充当转录终结的应激反应调节者.

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