在固态培养中,通过Aspergillus protuberus从发酵的米中提取β-葡萄糖酶的液方法
P Suresh Yadav1, B V Siva Prasad1, M Subhosh Chandra2
1Department of Microbiology, Yogi Vemana University, Kadapa, Andhra Pradesh, India.
Current microbiology
|April 15, 2024
概括
农业废物转化为宝贵的产品,如β-葡萄糖酶至关重要. 研究人员使用乙酸缓冲剂优化了从大米中提取酶,为工业应用实现了高产量.
科学领域:
- 生物技术是生物技术.
- 环境科学 环境科学
- 酶工程是什么? 酶工程是什么?
背景情况:
- 农业废物处理在发展中国家带来了环境挑战.
- 像β-葡萄糖酶这样的酶对于将废物转化为有价值产品至关重要.
- β-葡萄糖酶在食品,织品和生物燃料等行业具有多样化的应用.
研究的目的:
- 通过固态发酵在米上使用Aspergillus protuberus产生β-葡萄糖酶.
- 为了优化提取溶剂和条件,最大限度地恢复β-葡萄糖酶.
主要方法:
- 米的固态发酵 (SSF) 是由阿斯伯吉路斯 (Aspergillus protuberus) 进行的.
- 选各种溶剂以检测β-葡萄糖酶出效率.
- 提取参数的优化,包括缓冲器类型,pH值,体积和洗周期.
主要成果:
- 乙缓冲剂 (0.02 M,pH 5.0) 被确定为最有效的溶剂.
- 优化提取涉及两次洗用乙缓冲剂 (1g米:5ml缓冲剂) 每次30分钟.
- 获得的最大β-葡萄糖酶恢复率为41.95U/g米.
结论:
- 有效的β-葡萄糖酶生产和从大米中提取是可行的,使用阿斯伯吉路斯突起体和优化的酸盐缓冲漏.
- 这种方法提供了一种可持续的方法来利用农业废物并获得工业相关的酶.
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