优化微生物反应器培养策略Trichoderma reesei:从批量到料批量操作
Katja Rohr1,2, Lisa Gremm1,2, Bertram Geinitz1
1Institute of Bio- and Geosciences, IBG-1: Biotechnology, Forschungszentrum Jülich GmbH, Wilhelm-Johnen-Straße, 52428, Jülich, Germany.
Microbial cell factories
|April 15, 2024
概括
这项研究开发了Trichoderma reesei的微生物反应器培养方案,增强细胞酶的产生. 微型料批量策略显著提高了酶活性,为工业生物技术提供了有价值的工具.
科学领域:
- 生物技术是生物技术.
- 微生物学 微生物学
- 酵素工程是什么? 酶工程是什么
背景情况:
- 纤维状真菌,特别是Trichoderma reesei,是诸如纤维酶之类的工业酶的关键生产者.
- 由于复杂的形态,培养和表征T. reesei菌株是具有挑战性的,尽管有先进的基因编辑工具.
研究的目的:
- 为生产细胞酶的T. reesei菌株开发一个高通量微生物反应器培养协议.
- 探索微量料批量种植,以优化工业酶生产条件.
主要方法:
- 开发并验证了使用BioLector微生物反应器,适应中高频率的批量培养协议.
- 建立了一个微量食批量策略,使用乳糖作为双重用途的诱导剂和碳源.
- 与细胞干重相关联的分散光强度,以监测真菌生物质形成.
主要成果:
- 成功建立了T. reesei强大的批量栽培协议,克服了形态挑战.
- 在分散光强度和真菌生物量之间显示出强烈的相关性.
- 通过微尺度食批量培养与批量过程相比,实现了三倍增长的细胞氧化酶和五倍增长的β-葡萄糖酶活性.
结论:
- 微生物反应器的工作流程有效地优化T. reesei细胞酶的生产和菌株选.
- 微型料批量策略显示出在工业生物技术中增强酶生产的巨大潜力.
- 开发的协议为评估生物过程参数和推进酶生产应用提供了有价值的工具.
相关概念视频
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01:22Bioreactor Controls-III
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01:14Scale-Up Processes
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01:27Upstream Processing
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01:27Production of Antibiotics
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