Wnt信号调节了循环的人类单细胞的化学生产和细胞迁移
Natalie Zelikson1, Shaina Ben1, Michal Caspi1
1Department of Clinical Microbiology and Immunology, Faculty of Medical & Health Sciences, Tel Aviv University, Tel Aviv, Israel.
Cell communication and signaling : CCS
|April 15, 2024
概括
对于平衡至关重要的Wnt信号通路对炎症和癌症产生影响. 这项研究揭示了Wnt-3a刺激单细胞,通过CCL2增强它们的迁移,为炎症性疾病提供了洞察力.
科学领域:
- 免疫学 免疫学 免疫学
- 分子生物学分子生物学
- 细胞信号传递 细胞信号传递
背景情况:
- 规范的Wnt信号通路由β-catenin调节,对于细胞平衡至关重要.
- Wnt信号的失调与炎症性疾病和癌症有关.
- 单细胞在炎症反应中起着关键作用.
研究的目的:
- 调查Wnt信号在人体初级单细胞中的作用.
- 探索Wnt-3a刺激对单细胞功能的影响.
- 了解炎症性疾病 (如类风湿性关节疾病) 的影响.
主要方法:
- 在循环的人类单细胞中分析Wnt信号组件.
- 用Wnt-3a刺激单细胞,并评估β-catenin和基因表达.
- 测量细胞因子和化学因子分泌和单细胞迁移.
- 从患有风湿性关节疾病的患者的血液样本进行活体实验.
主要成果:
- 循环的人类单细胞表达Wnt信号元件并响应Wnt-3a.
- 刺激Wnt-3a可以增加β-catenin水平,但不能增加Wnt目标基因表达.
- Wnt-3a诱导细胞因子/化学因子分泌,并增强单细胞迁移,由CCL2.
- 来自风湿关节疾病患者的单细胞在Wnt-3a治疗时表现出明显的细胞因子配置.
结论:
- Wnt-3a作为单细胞免疫反应的强有力的刺激剂,包括迁移.
- 这突显了Wnt信号在调节白细胞炎症过程中的新作用.
- 这些发现有助于理解Wnt通路在炎症疾病和免疫细胞功能中的参与.
相关概念视频
Canonical Wnt Signaling Pathway
8.7K
The gene encoding the main signaling molecules of the Wnt signaling pathways (the Wnt proteins) was discovered almost four decades ago by Nüsslein-Volhard and Wieschaus. They identified and originally named the gene "wingless" (wg) after a phenotype discovered during their landmark genetic screen in Drosophila for body pattern defects. At around the same time, another researcher named Harold Varmus found that a murine tumor virus activates the mammalian wg homolog, Int-1, which...
8.7K
Non-Canonical Wnt Signaling Pathways
7.3K
Wnt is a zygotic effect gene that is expressed during very early embryonic development. It regulates various processes in animals starting from early development through the adult stage, such as organogenesis in the embryo and maintenance of neuronal and blood stem cells. Wnt proteins can induce a wide variety of intracellular pathways depending upon the specific abilities of different Wnt ligands to form a complex with shared and cognate receptors in the presence of different co-receptors. The...
7.3K
Differentiation of Common Myeloid Progenitor Cells
3.2K
Common myeloid progenitors (CMPs) are oligopotent cells that can differentiate into granulocytes and macrophages. Granulocytes and macrophages are essential for protecting the body against bacterial, viral, or fungal infections. They migrate from the bone marrow into the circulating blood to reach specific tissue sites where they differentiate and help in immune surveillance. However, they survive only for a few days and must be continuously made available to the organism to maintain a robust...
3.2K
Chemotaxis and Direction of Cell Migration
3.4K
Cells can detect chemical cues in their environment and reorganize the cytoskeleton to migrate toward them or away from them. This directional migration, called chemotaxis, is essential during embryogenesis and development, immune response, tissue repair and regeneration, and reproduction. These chemical cues can either attract or repel the cell's movement. For example, axon development is determined by a combination of chemoattractants and chemorepellents that direct the growing axon...
3.4K


