概括
自动化血液学分析仪可以提供不准确的血小板计数,即使没有标志或异常组图. 这一案例凸显了需要多种测试方法,包括显微镜,以准确评估复杂患者的血小板.
科学领域:
- 血液学 血液学 血液学
- 临床病理学 临床病理学
- 实验室医学 实验室医学
背景情况:
- 自动化血液学分析仪对于血小板 (PLT) 计数的确定至关重要.
- 虚假的PLT结果可能导致错误的医疗判断和重大风险.
- 异常的PLT组图和标志通常会触发对虚假结果的审查.
研究的目的:
- 报告在血造细胞干细胞移植后严重的无形成性贫血中虚假高血小板计数的病例.
- 调查一个标准审查标准 (组图,标志) 无法检测不准确的血小板计数的情况.
主要方法:
- 使用Sysmex XN系列分析仪 (电阻 - PLT-I,光 - PLT-F) 进行血小板计数.
- 在MINDRAY BC-7500分析仪上重新测试样本,并进行了冷球蛋白干扰测试.
- 使用手动显微镜估计血小板数量和评估细胞形态.
主要成果:
- 无论是PLT-I还是PLT-F都产生了虚假的高血小板计数.
- 显微镜评估提供了更可靠的血小板计数.
- 血液溶解诱导的棘状和幽灵细胞可能导致仪器计数不准确.
结论:
- 使用PLT-I (即使有标志) 和PLT-F进行血小板计数,可能不足以查复杂的血液病例.
- 手动显微镜对于在特定患者群体中准确的血小板评估至关重要.
- 为了可靠的血小板量化,建议使用多种测试方法.
相关概念视频
01:27Disorders of Leukocytes
Leukocyte disorders can lead to either leukopenia, characterized by an abnormally low leukocyte count, or leukocytosis, marked by a very high leukocyte number.
Leukopenia may result from bone marrow disorders, autoimmune diseases, and infectious diseases. For example, conditions such as multiple myeloma and aplastic anemia can impair the bone marrow's ability to produce adequate leukocytes. Similarly, autoimmune diseases like lupus and viral infections such as HIV can prompt the immune system...
Leukopenia may result from bone marrow disorders, autoimmune diseases, and infectious diseases. For example, conditions such as multiple myeloma and aplastic anemia can impair the bone marrow's ability to produce adequate leukocytes. Similarly, autoimmune diseases like lupus and viral infections such as HIV can prompt the immune system...
01:18Structure and Function of Platelets
The cell fragments known as platelets are disc-shaped, with an average diameter of about 3 μm and a thickness of roughly 1 μm. They play a crucial role in the body's vascular clotting system, which also involves plasma proteins, blood cells, and blood vessel tissues.
Platelets are continually replenished, circulating in the bloodstream for 9-12 days before being removed by phagocytes, primarily in the spleen. A microliter of circulating blood contains between 150,000 and 450,000 platelets, with...
Platelets are continually replenished, circulating in the bloodstream for 9-12 days before being removed by phagocytes, primarily in the spleen. A microliter of circulating blood contains between 150,000 and 450,000 platelets, with...