在固定和表皮质结合之间走一条细线 - 适用于乙素和HSV-2染色的抗原检索方法的表征,用于甲固定女性生殖组织中的雌激素染色

Immunoelectron microscopy utilizes immunogold labeling of endogenous proteins with specific antibodies to detect and localize these proteins in cells and tissues. The procedure provides insights into the distribution and quantification of protein under different stimulation conditions offering clues about their functions. Conjugating highly electron-dense gold particles with primary or secondary antibodies allow antigen detection on and within cells, with high resolution and specificity.