旧的角色与新的特征:T2SS ATPase作为循环-di-GMP受体来调节抗生素生产
Haofei Liu1, Gaoge Xu2,3, Baodian Guo2
1College of Plant Protection, Nanjing Agricultural University, Nanjing, China.
Applied and environmental microbiology
|April 16, 2024
概括
研究人员确定了LspE,一种II型分泌系统ATPase,作为细菌中的循环二-GMP (c-di-GMP) 受体. 这种蛋白还通过不同的c-di-GMP结合和ATPase活动来调节抗真菌抗生素HSAF的产生.
科学领域:
- 细菌分子生物学 细菌分子生物学
- 第二个信使的信号通道.
- 蛋白质 - 配体相互作用
背景情况:
- 循环二-GMP (c-di-GMP) 是一种重要的细菌第二信使,调节各种细胞过程.
- 鉴定c-di-GMP受体是具有挑战性的,因为蛋白质的结构多样性.
- 二型分泌系统 (T2SS) ATPase GspE 是一种特征很好的细菌蛋白质.
研究的目的:
- 在细菌中识别新的c-di-GMP受体蛋白.
- 描述LspE的功能,这是来自*Lysobacter enzymogenes*的GspE同源,作为c-di-GMP受体.
- 调查LspE在调节抗真菌抗生素HSAF生产中的作用.
主要方法:
- 生物信息分析以确定LspE.中的潜在c-di-GMP结合残留物.
- 生物化学分析以确认LspE.的c-di-GMP结合和ATPase活性.
- 基因操纵以评估LspE对L. enzymogenes*中HSAF产生的影响.
主要成果:
- LspE被确定为c-di-GMP受体,具有关键结合残留物K358和T359.
- LspE在调节抗真菌抗生素HSAF的产生方面表现出一种新的功能.
- LspE对HSAF的调节涉及独立的c-di-GMP结合和ATPase活动.
结论:
- T2SS ATPase GspE代表了一个保存的c-di-GMP受体蛋白质类.
- 在细菌生理学中,LspE扮演着双重角色,既是c-di-GMP受体,又是抗生素生物合成的调节者.
- 这项研究扩展了T2SS组件的已知功能,并突出了c-di-GMP信号研究的新途径.
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