从造血细胞原生细胞和诱导多能干细胞 (iPSCs) 中性粒细胞的产生:潜在的应用
Abdollah Jafarzadeh1, Marzieh Motaghi2, Sanand Kumar Patra3
1Applied Cellular and Molecular Research Center, Kerman University of Medical Sciences, Kerman, Iran; Department of Immunology, School of Medicine, Kerman University of Medical Sciences, Kerman, Iran; Clinical Immunology Research Center, Zahedan University of Medical Sciences, Zahedan, Iran.
Cytotherapy
|April 16, 2024
概括
诱导多能干细胞 (iPSCs) 可以产生中性粒细胞,这些免疫细胞对抗感染至关重要. 本综述涵盖了从干细胞生成中性粒细胞的方法及其研究应用.
科学领域:
- 免疫学 免疫学 免疫学
- 干细胞生物学 干细胞生物学
- 细胞分化 细胞分化
背景情况:
- 中性粒细胞是重要的免疫细胞,缺陷导致严重的感染.
- 造血原生细胞 (HPC) 和胚胎干细胞 (ESC) 可以产生中性粒细胞.
- 诱导多能干细胞 (iPSC) 提供了一种可再生的来源,用于产生各种细胞类型,包括中性粒细胞.
研究的目的:
- 审查从HPC,ESC和iPSC生成中性粒细胞的协议.
- 讨论中性粒细胞生成在研究和医学中的应用.
- 要突出在iPSC衍生中性粒细胞的治疗应用中的挑战.
主要方法:
- 来自iPSC的中性粒细胞生成涉及多步骤的过程,可以适应依赖料或独立系统.
- 细胞因子,如干细胞因子,IL-3,血栓形成素和G-CSF等细胞因子对于分化至关重要.
- 颗粒细胞殖民地刺激因子 (G-CSF) 对于最终的中性粒细胞成熟尤为重要.
主要成果:
- 来自iPSC的中性粒细胞是研究遗传中性粒细胞疾病的宝贵工具.
- 这些细胞适用于疾病建模,感染研究和药物发现.
- 目前的协议允许从各种干细胞来源生成中性粒细胞.
结论:
- iPSC技术为研究目的中性粒细胞生成提供了一个有前途的途径.
- 需要进一步的进展,以克服iPSC衍生的中性粒细胞的治疗移植的挑战.
- 来自干细胞的中性粒细胞产生具有促进免疫学和再生医学的巨大潜力.
相关概念视频
01:22iPS Cell Differentiation
2.7K
The ability of induced pluripotent stem cells or iPSCs to differentiate into most body cell types has stimulated repair and regenerative medicine research over the past few decades. iPSC-derived blood cells, hepatocytes, beta islet cells, cardiomyocytes, neurons, and other cell types can repair injuries or regenerate damaged tissue in diseases such as diabetes and neurodegenerative disorders.
2.7K
01:13Induced Pluripotent Stem Cells
22.7K
Stem cells are undifferentiated cells that divide and produce different types of cells. Ordinarily, cells that have differentiated into a specific cell type are post-mitotic—that is, they no longer divide. However, scientists have found a way to reprogram these mature cells so that they “de-differentiate” and return to an unspecialized, proliferative state. These cells are also pluripotent like embryonic stem cells—able to produce all cell types—and are therefore...
22.7K
01:17Stem Cell Culture
5.2K
Stem cell research aims to find ways to use stem cells to regenerate and repair cellular damage. Over time, most adult cells undergo the wear and tear of aging and lose their ability to divide and repair themselves. Stem cells do not display a particular morphology or function. Adult stem cells, which exist as a small subset of cells in most tissues, keep dividing and can differentiate into a number of specialized cells generally formed by that tissue. These cells enable the body to renew and...
5.2K
01:19Multipotency of Hematopoietic Stem Cells
3.1K
The hematopoietic stem cells or HSCs are multipotent, meaning they can differentiate and give rise to all blood and immune cells. HSCs are maintained in the quiescent stage until an external stimulus initiates their differentiation. The multipotent HSCs exist as two heterogeneous populations, long-term repopulating cells (LTRC) and short-term repopulating cells (STRC). The two HSC populations have different surface markers or receptors and are classified based on quiescence and long-term...
3.1K
01:27Source And Potency Of Stem Cells
4.8K
Stem cells are undifferentiated cells with extensive self-renewal properties that help them maintain their population during the fetal and adult stages of life. They can specialize in all cell types of the human body. However, their differential potential may vary and can be classified into five types. Stem cells can be (1) Totipotent, (2) Pluripotent, (3) Multipotent, (4) Oligopotent, and (5) Unipotent. Each stem cell has a specific origin; the fertilized egg or zygote is a totipotent cell and...
4.8K
01:21EPS and iPS Cells in Disease Research
2.8K
Embryonic and induced pluripotent stem cells are excellent models for disease research because of their ability to self-renew and differentiate into most cell types. Somatic cells from a patient are isolated and reprogrammed into induced pluripotent stem cells or iPSCs. These iPSCs are later differentiated into the desired cell type, which mirrors the diseased cell of the patient. In this way, disease models have been created for investigating diseases such as Down syndrome, type I diabetes,...
2.8K