卡德林-17作为腺癌瘤免疫oPET的标
Samantha Delaney1,2,3, Outi Keinänen4, Dennis Lam5
1Department of Chemistry, Hunter College of the City University of New York, 413 East 69th Street, New York, NY, 10021, USA.
European journal of nuclear medicine and molecular imaging
|April 16, 2024
概括
一种新型的-89标记的针对卡德林-17 (CDH17) 的免疫PET探针显示,它有望用于可视化胰腺管道腺癌 (PDAC). 这种针对CDH17的探针可实现非侵入性成像,可能有助于胃肠道癌症的治疗应用.
科学领域:
- 在瘤学瘤学.
- 放射化学 放射化学是指辐射化学.
- 分子成像学分子成像学
背景情况:
- 卡德林-17 (CDH17) 在胃,结肠直肠和胰腺腺癌中过度表达.
- 升高的CDH17水平与转移和患者预后不佳相关.
- CDH17是新型诊断和治疗策略的潜在目标.
研究的目的:
- 为了合成和评估一种新的Zr标记的免疫oPET探针,准CDH17.
- 评估CDH17向探针的体外和体内性能.
主要方法:
- 使用desferrioxamine (DFO) 修改CDH17向的mAb D2101以创建DFO-D2101.
- 用89Zr对DFO-D2101进行放射性标记,并评估血清稳定性和免疫活性.
- 在小鼠模型中使用PET成像和生物分布研究对胰腺管腺癌 (PDAC) 的体内评估.
主要成果:
- 免疫结合物DFO-D2101保留了与母 mAb.Ab.类似的抗原结合特性.
- 标有Zr标记的探针[Zr-DFO-D2101]显示出良好的血清稳定性和高免疫活性.
- 该探测器成功地在PDAC模型中可视化了CDH17表达瘤,具有高对比度和低的目标外吸收.
结论:
- [89Zr]Zr-DFO-D2101是一种有前途的PET探针,用于在PDAC中非侵入性地可视化CDH17表达.
- 这种放射性免疫结合物可能会显著影响PDAC和胃肠腺癌的临床管理.
- 该探针具有作为一种超神论工具的潜力,以支持针对CDH17的治疗方法.
相关概念视频
Structure of Cadherins
The cadherins were one of the first cell adhesion molecules discovered; the term “cadherins” is based on their calcium-dependent adhering properties. The first cadherins discovered on the epithelial, neuronal, and placental cells were named E-cadherin, P-cadherin, and N-cadherin, respectively. These classical cadherins share sequence and structural similarities. Other cadherins, including those involved in cell signaling, are grouped into non-classical cadherins. This diversity of cadherins...
Adherens Junctions
Strong contact points between adjacent cells anchor them to each other, forming tissues. Such anchoring junctions are of two types – adherens junctions and desmosomes. Adherens junctions are abundant in tissues such as epithelium and endothelium, forming a continuous zone of adhesion called the adhesion belt. In other tissues, such as heart muscle, they appear as clusters, linking the cells to produce coordinated heart muscle contraction.
Adherens Junctions are Dynamic
The endothelial cells...
Adherens Junctions are Dynamic
The endothelial cells...
Cadherins in Tissue Organization
The cadherins are a superfamily of cell adhesion molecules comprising over 180 variants, with specific tissues expressing a particular combination of cadherin types. Cadherins generally exhibit homophilic binding; i.e., cadherins on one cell bind to cadherins of the same or closely related type on another cell. Thus, cells of the same type have a specific affinity to bind to each other and sort themselves into clusters to form tissues.
Cell Sorting During Development
Cell sorting plays an...
Cell Sorting During Development
Cell sorting plays an...
Immunoglobulin-like Cell Adhesion Molecules
Immunoglobulin-like cell adhesion molecules or Ig-CAMs are a versatile group of cell surface glycoproteins belonging to the immunoglobulin protein superfamily. Ig-CAMs possess the characteristic immunoglobulin protein domains and other domains such as the fibronectin type III domain. The Ig domains are glycosylated to varying degrees in different Ig-CAMs.
Ig-CAMs exhibit either homophilic binding (to other Ig-CAMs) or heterophilic binding (to other ligands such as integrins). While most Ig-CAMs...
Ig-CAMs exhibit either homophilic binding (to other Ig-CAMs) or heterophilic binding (to other ligands such as integrins). While most Ig-CAMs...


