杆菌 (Pectobacterium araliae) sp. 在这些细菌中 一种致病原体,导致日本日本树的细菌软腐烂
Hiroyuki Sawada1, Nobutaka Someya2, Tomohiro Morohoshi3
1Research Center of Genetic Resources, National Agriculture and Food Research Organization (NARO), 2-1-2 Kannondai, Tsukuba, Ibaraki 305-8602, Japan.
概括
一种新型的细菌物种,Pectobacterium araliae,从遭受细菌软腐烂的日本天使树中鉴定出来. 这种植物病原菌代表了Pectobacterium属的新增,影响了植物健康.
科学领域:
- 微生物学 微生物学
- 植物病理学 植物病理学
- 细菌学 细菌学是一门学科.
背景情况:
- 植物病原菌在全球范围内对农作物和树木造成重大破坏.
- 细菌软腐病,特别是木质植物中的软腐病,对植物健康和生物多样性构成威胁.
- 百科细菌属包括几个致病植物物种,它们负责引起软腐烂疾病.
研究的目的:
- 描述和分类从日本天使树中分离出来的新型细菌菌株,这些细菌菌株表现出细菌软腐烂的症状.
- 为了确定这些分离物在Pectobacterium属内的遗传学和分类学位置.
- 根据全面的表型,化学分类学和基因型分析,提出一种新的物种名称.
主要方法:
- 从患病的植物组织中分离和培养细菌.
- 格拉姆染色,运动性测试和子形成测试.
- 基因组DNA G+C含量确定和细胞脂肪酸分析.
- 使用16S rRNA和gyrB基因序列进行了家族遗传学分析.
- 全基因组测序用于基因组学分析.
- 基因组比较分析包括平均核酸标识 (ANI) 和数字DNA-DNA杂交 (dDDH).
- 表型特征和与相关物种的比较.
主要成果:
- 两种细菌菌株MAFF 302110T和MAFF 302107从具有细菌软腐烂的日本天使树中分离出来.
- 这些分离物被确定为Gram阴性,选择性无氧,运动棒,非子形成.
- 遗传学分析始终将这些菌株置于Pectobacterium属内,但与已知的物种不同.
- ANI和dDDH值低于物种划分的确定的值,其中Pectobacterium polonicum DPMP315T显示出最高的相似性.
- 独特的表型特征,脂肪酸特征和分泌系统使这些分离物与相关物种有所区别.
结论:
- 综合的表型,化学分类学和基因型数据强烈支持将MAFF 302110T和MAFF 302107分类为Pectobacterium属内的新物种.
- 这种新物种的建议名称是Pectobacterium araliae sp. nov.,其中MAFF 302110T被指定为类型菌株.
- 这一发现扩大了已知的Pectobacterium物种的多样性,并有助于了解影响日本天使树的病原体.
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