精细地图和两种棕色植物抗性基因的育种应用,这些基因来自陆地米
Fahuo Li1,2, Liuhui Yan1,3, Juan Shen1,2
1College of Agriculture, Guangxi Key Laboratory of Agro-environment and Agric-Products Safety, State Key Laboratory for Conservation and Utilization of Subtropical Agro-bioresources, Guangxi University, Nanning, China.
PloS one
|April 16, 2024
概括
研究人员在米陆地品种中发现了新的棕色植物 (BPH) 抵抗基因. 这些发现支持通过标记器辅助选择开发耐昆虫的米品种,以改善作物保护.
科学领域:
- 植物育种 植物育种
- 遗传学 遗传学 是一个
- 农业科学 农业科学
背景情况:
- 棕色 (Nilaparvata lugens Stål; BPH) 在亚洲造成大米作物的重大破坏.
- 抗宿主米品种对于环保的BPH控制至关重要.
- 兰德雷斯大米种群拥有多样化的BPH抗性基因,对育种计划有价值.
研究的目的:
- 为了识别和绘制印加大米CL45和CL48.8陆地品种的新型BPH耐药位点.
- 在BPH耐药性繁殖中开发用于标记器辅助选择 (MAS) 的分子标记物.
- 探索潜在的候选基因,这些基因是BPH抵抗的基础.
主要方法:
- 使用分子标记物 (例如,12M16.983,12M19.042,RM26567,11MA104) 来识别大米染色体上的抗性位点的遗传映射.
- 精确地绘制已识别的抵抗位点,以缩小基因组间隔.
- 使用MAS开发近同位素线 (NIL) 和金字塔线.
- 在发达品种中对抗性的表型评估.
主要成果:
- 一个新的抵抗位点,Bph46,被确定并映射到CL45中的染色体12上,精确地映射到480 kb间隔.
- 另一种抵抗位点,qBph11.3,被确定并映射到CL48中的染色体11上,精细地映射到145 kb间隔.
- 分子标记12M16.983和11MA104分别为CL45和CL48开发.
- 发现CL48中的黑色色与BPH耐药性密切相关.
- 开发的NIL和金字塔线显示出对BPH的显著高耐药性.
结论:
- 两种新的BPH耐药位点,Bph46和qBph11.3,在米陆地品种中成功地绘制了地图.
- 已开发的分子标记物和已识别的相关特征 (黑色色) 促进了BPH耐药性繁殖的高效MAS.
- 这项研究提供了宝贵的遗传资源和策略,用于开发耐用的抗BPH米品种.
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