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在Candida albicans中确定一个活跃的RNAi通路

Elise Iracane¹, Cristina Arias-Sardá¹, Corinne Maufrais²

¹Kent Fungal Group, School of Biosciences, Division of Natural Sciences, University of Kent, Canterbury CT2 7NZ, United Kingdom.

Proceedings of the National Academy of Sciences of the United States of America

|April 16, 2024

概括

在大多数Candida albicans菌株中,RNA干扰 (RNAi) 途径是功能性的,与常用的基准菌株有突变相反. 这一发现影响了对这种重要的真菌病原体的研究.

科学领域:

微生物学微生物学
分子生物学分子生物学
遗传学是一个遗传学.

背景情况:

RNA干扰 (RNAi) 是一种在真核生物中发现的关键基因调节途径.
虽然在真菌中很常见,但一些物种如Saccharomyces cerevisiae缺乏功能性RNAi.
作为人类重要病原体的Candida albicans缺乏已证明的RNAi活性.

研究的目的:

为了研究Candida albicans中RNAi通路的存在和功能.
在不同的C. albicans菌株中识别影响RNAi的遗传变异.
了解RNAi在C. albicans.基因表达调节中的作用.

主要方法:

高通量小型和长RNA测序.
基于CRISPR/Cas9的基因编辑.
在各种C. albicans分离物中分析阿尔戈诺特基因.

主要成果:

标准的C. albicans参考菌株 (SC5314) 在它的阿尔戈诺特基因中存在无活化突变.
大多数其他C. albicans分离物具有功能性的阿尔戈诺特蛋白,表明有活跃的RNAi机制.
在C. albicans中,活性RNAi抑制了亚端粒基因家族的表达.

结论:

在大多数Candida albicans菌株中存在一个功能性RNAi通路.
常见的参考菌株SC5314并不代表C. albicans中典型的RNAi状态.
研究人员应该利用各种C. albicans菌株来准确研究这种病原体.

关键词:

这是一种RNA干扰.菌类病原体的真菌病原体这些子端粒是分端粒.

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