黄金葡萄球菌通过抑制Rab11-Rab11FIP4介导的囊泡贩运来促进其细胞内生存
Huiling Xu1, Xiaozhou Wang1, Zhizhong Zhang1
1College of Veterinary Medicine, Shandong Agricultural University, Tai`an, Shandong 271018, China.
Veterinary microbiology
|April 16, 2024
概括
Rab11A有助于牛乳腺上皮细胞清除黄金葡萄球菌,这是一个常见的乳腺炎原因. 相反,Rab11fip4阻碍了这种细菌清除,影响宿主细胞防御.
科学领域:
- * 乳牛的健康状况
- * 细菌病原发生学
- *细胞免疫学 细胞免疫学
背景情况:
- * 乳牛乳腺炎通常是由金黄色葡萄球菌引起的.
- *乳腺上皮细胞构成了对乳腺炎的关键物理屏障.
- * 之前的研究表明,Rab11fip4因S. aureus刺激而改变.
研究的目的:
- * 评估Rab11A在牛乳腺上皮细胞S. aureus的细胞化中的作用.
- * 研究Rab11A和Rab11fip4对黄金色细菌的增殖和宿主细胞防御机制的影响.
主要方法:
- * 免疫光和西部抹影用于分析Rab11A和Rab11fip4的表达.
- *评估了对细菌增殖,晚期内分体 (LE) 和 lysosome (LYS) 形成以及功能的影响.
- *观察到Rab11A和Rab11fip4对含有黄金色杆菌的虫体的招募.
主要成果:
- *Rab11A和Rab11fip4都被招募到感染后含有金黄色细菌的胞体中.
- * Rab11A促进了黄金菌的清除,并保护了LEs和LYS免受细菌损伤.
- * Rab11fip4表现出相反的效果,阻碍细菌清除并损害LEs和LYSs.
结论:
- *Rab11A在牛乳腺上皮细胞中起着保护性作用,防止S. aureus感染.
- * Rab11fip4似乎通过损害宿主细胞防御来促进金黄色细菌的致病.
- *研究结果提供了对黄金菌细胞和宿主细胞控制的见解,有助于乳腺炎研究.
相关概念视频
Rab Cascades
2.6K
Rab GTPases act in a regulated cascade during membrane fusion, helping the lipid bilayers mix. The Rab family of proteins are active when bound to GTP, and inactive when bound to GDP. Hence, they act as guanine nucleotide-dependent molecular switches. Rab-GTP recognizes and binds to long or short-range tethering proteins to capture the target vesicle. These tethers coordinate with SNAREs on the vesicle and the target membrane to assemble the trans SNARE complex that locks the mixing bilayers.
2.6K
Rab Proteins
3.9K
Rab proteins constitute the largest family of monomeric GTPases, of which 70 members are present in humans. Rab proteins and their effectors regulate consecutive stages of vesicle transport such as vesicle transport, docking, and fusion to the correct recipient membrane.
Rab proteins switch between a cytosolic, GDP-bound inactive state and a membrane-anchored, GTP-bound active state. By themselves, Rabs show slow rates of GDP/GTP exchange and GTP hydrolysis. Thus, Rab proteins are considered...
Rab proteins switch between a cytosolic, GDP-bound inactive state and a membrane-anchored, GTP-bound active state. By themselves, Rabs show slow rates of GDP/GTP exchange and GTP hydrolysis. Thus, Rab proteins are considered...
3.9K
SNAREs and Membrane Fusion
10.9K
Once a transport vesicle has recognized its target organelle, the vesicular membrane needs to fuse with the target membrane to unload the cargo. Transmembrane proteins called SNAREs present on organelle membranes and their vesicles, mediate vesicle fusion.
SNAREs exist in pairs that symmetrically interact and catalyze the fusion of the lipid bilayers in vesicle and target organelle. v-SNARE in the vesicle membrane are single polypeptide chains that bind to a complementary t-SNARE, composed of 2...
SNAREs exist in pairs that symmetrically interact and catalyze the fusion of the lipid bilayers in vesicle and target organelle. v-SNARE in the vesicle membrane are single polypeptide chains that bind to a complementary t-SNARE, composed of 2...
10.9K
Recycling Endosomes and Transcytosis
2.7K
The recycling endosome, also known as the endosomal recycling compartment (ERC), is a part of the slow-recycling process of the endocytic pathway. Molecules internalized through receptor-mediated endocytosis are either degraded in the lysosomes or are recycled to the plasma membrane through the fast- or slow-recycling route.
The recycling endosome is not a single organelle but an extensively tubulated network of recycling pathways. It functions in storing molecules or transporting them across...
The recycling endosome is not a single organelle but an extensively tubulated network of recycling pathways. It functions in storing molecules or transporting them across...
2.7K
Overview of Secretory Vesicles
8.5K
Secretory vesicles, also known as dense core vesicles (DCVs), are membrane-bound vesicles that transport secretory proteins, such as hormones or neurotransmitters. Regulated secretory vesicles transport proteins from the trans-Golgi network to the exterior of the cell. Proteins present in regulated secretory vesicles are required to be rapidly exocytosed in large amounts upon a specific stimulus.
Various proteins regulate the aggregation of molecules inside the secretory vesicles. Chromogranins...
Various proteins regulate the aggregation of molecules inside the secretory vesicles. Chromogranins...
8.5K
Pinching-off of Coated Vesicles
3.1K
Vesicle budding is orchestrated by distinct cytosolic proteins such as adaptor proteins, coat proteins, and GTPases. To initiate vesicle budding, membrane-bending proteins containing crescent-shaped BAR domains bind to the lipid heads in the bilayer and distort the membrane to form a protein-coated vesicle bud. Adaptors proteins such as AP2 for clathrin-coated vesicles can nucleate on the deformed membrane. Finally, coat proteins such as clathrin or COPI and COPII assemble into a coat forming...
3.1K


