MUC4 O-GlcNAcylation 调节结膜上皮细胞中的上皮表型
Wei-Wei Xiong1, Zi-Jing Ouyang1, Yi Tan1
1Department of Ophthalmology, The Second Affiliated Hospital, Jiangxi Medical College, Nanchang University, NO.1 Minde Road, Nanchang, 330006, Jiangxi, China.
Biochemical genetics
|April 16, 2024
概括
介质素-4 (IL-4) 影响眼细胞中的MUC4和O-GlcNAc转移酶 (OGT) /酶 (OGA). MUC4调节细胞连接蛋白,提供了对过敏结膜炎病原体的见解.
科学领域:
- 眼科医生 眼科 眼科
- 细胞生物学 细胞生物学
- 免疫学 免疫学 免疫学
背景情况:
- 过敏性结膜炎 (AC) 涉及眼睛炎症.
- 菌素在眼睛表面的健康方面起着重要作用.
- 洲际蛋白-4 (IL-4) 是过敏反应中的一个关键细胞因子.
研究的目的:
- 研究MUC4在受IL-4刺激的人类结膜上皮细胞 (HConEpic) 中的作用.
- 阐明涉及O-GlcNAc转移酶 (OGT) 和O-GlcNAc酶 (OGA) 的基本机制.
- 评估对关键细胞结 protein 的影响.
主要方法:
- 用人类重组IL-4治疗HConEpic细胞.
- 使用MUC4的shRNA (sh-MUC4) 来抑制MUC4的表达.
- 通过Western blot分析了MUC4,OGT,OGA,ZO-1,GJB2,CLDN8和E-cadherin的蛋白质水平.
- 同免疫沉 (Co-IP) 评估了MUC4,OGT和OGA之间的相互作用.
主要成果:
- 在HConEpic细胞中,IL-4对MUC4和OGT进行了上调,同时对HConEpic细胞中的OGA进行了下调.
- IL-4降低了ZO-1,GJB2,CLDN8和E-cadherin的水平,这些影响被sh-MUC4逆转.
- OGT抑制降低了MUC4和增加了接口蛋白;OGA抑制具有相反的效果.
- MUC4 O-GlcNAcylation被OGT/OGA 抑制剂改变,影响了MUC4-OGT/OGA 相互作用.
结论:
- IL-4影响结膜上皮细胞中的MUC4和OGT/OGA通路.
- 由OGT/OGA调节的MUC4影响细胞结点蛋白表达.
- 这些发现提高了对过敏结膜炎病原体的理解.
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