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来自南极的冷适应素样蛋白酶的表征和水解机制分析

Shanglin Xin^1,2,1, Haiyang Zhang^1,2,1, Jianan Sun^1,2,1

¹Key Laboratory of Biological Processing of Aquatic Products, China National Light Industry, Qingdao 266404, PR China.

Journal of agricultural and food chemistry

|April 17, 2024

概括

研究人员鉴定了南极的适应寒冷的素 (OUC-Pp-20),发现它在低温下有效. 这种酶通过降解过敏原和特定蛋白质,显示出低温食品加工的潜力.

科学领域:

生物化学生物化学
食品科学食品科学食品科学
酶学是一种酶学.

背景情况:

适应寒冷的蛋白酶在降低温度下提供有效的蛋白质水解,这对于低温食品加工非常有价值.
南极具有高度活跃的自溶性酶系统,对死后肌肉蛋白质分解至关重要.
素在南极的自溶过程中发挥着公认的关键作用.

研究的目的:

描述南极的适应寒冷的蛋白质酶,特别关注试素.
从南极鱼中克隆和表达一种适应寒冷的素 (OUC-Pp-20).
评估OUC-Pp-20在食品加工中的酶性质和潜在应用.

主要方法:

在 *Pichia pastoris* 中克隆和表达南极三素 (OUC-Pp-20).
确定复合素的分子量和最佳反应温度.
进行LC-MS/MS分析以确定OUC-Pp-20的催化特异性和水解部位.
在牛奶和肉上进行水解测定,以评估特定成分的降解.

主要成果:

重组OUC-Pp-20素是一种单体蛋白质,分子量为26.8±1.0kDa.
确定OUC-Pp-20的最佳反应温度为25°C.
在OUC-Pp-20中,在P1位置对Gln和Asn表现出特异性.
观察到OUC-Pp-20有效降解牛奶过敏原和的阿基因酶.

结论:

OUC-Pp-20是一种适应寒冷的素,具有特有的酶性质.
OUC-Pp-20的特异性和有效性表明它在低温食品加工应用中的实用性.
这些发现有助于了解适应寒冷的酶及其工业潜力.

关键词:

这就是Pichia pastoris.南极克里尔 (Antarctic Krill) 是南极的野生动物之一.适应了寒冷的素样蛋白酶.水解机制的水解机制.酸是一种酸.

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