在牛胎卵巢发育期间,素激素受体的表达
Alan Brunholi Giroto1, Marina Platzeck Chaves1, Priscila Helena Dos Santos2
1Universidade do Oeste Paulista, Presidente Prudente, SP, Brasil.
Animal reproduction
|April 17, 2024
概括
黄素激素受体 (LHCGR) 和其相关分子在正在发育的牛胎卵巢中得到表达. 这表明它们在调节牛早期毛囊发育中的关键作用.
科学领域:
- 生殖生物学 生殖生物学
- 分子内分泌学分子内分泌学
- 卵巢发育 卵巢发育
背景情况:
- 类固醇和淋巴激素调节毛囊发育.
- 化激素受体 (LHCGR) 的表达在其他物种中已知,但尚未在牛胎卵巢中证明.
- 了解LHCGR在早期牛卵泡发育中的作用至关重要.
研究的目的:
- 研究LHCGR和LHCGRmRNA结合蛋白 (LRBP) 在正在发育的牛胎卵巢中的表达.
- 在牛胎卵巢发育期间量化bta-miR-222,一个调节LHCGR的微RNA.
- 阐明控制牛早期毛囊发育的分子机制.
主要方法:
- 在不同妊娠阶段对LHCGR和LRBPmRNA丰度的定量分析.
- 在牛胎卵巢发育过程中量化bta-miR-222水平.
- 在各种卵巢细胞类型和卵泡阶段检测LHCGR蛋白的免疫组合化学检测.
主要成果:
- 在牛胎卵巢中检测到LHCGR表达,从 oogonia 到二次毛囊.
- LHCGR的mRNA丰度从妊娠60天降低到150天,而LRBP和bta-miR-222则显示出相反的趋势.
- 在早期毛囊的卵细胞和粒状细胞中局部化了LHCGR蛋白.
结论:
- 牛中LHCGR,LRBP和bta-miR-222都参与了前毛囊的发展.
- 这种分子复合体可能在调节早期卵巢卵泡生长方面发挥重要作用.
- 这些发现提供了关于牛卵泡发生的分子调节的见解.
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