Jove
Visualize
联系我们
JoVE
x logofacebook logolinkedin logoyoutube logo
关于 JoVE
概览领导团队博客JoVE 帮助中心
作者
出版流程编辑委员会范围与政策同行评审常见问题投稿
图书馆员
用户评价订阅访问资源图书馆顾问委员会常见问题
研究
JoVE JournalMethods CollectionsJoVE Encyclopedia of Experiments存档
教育
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab Manual教师资源中心教师网站
使用条款与条件
隐私政策
政策

相关概念视频

Raman Spectroscopy: Overview01:20

Raman Spectroscopy: Overview

373
The underlying principle of Raman spectroscopy is based on the interaction between light and matter, specifically molecules' inelastic scattering of photons. When a monochromatic beam of light, typically from a laser source, interacts with a sample, most scattered light has the same frequency as the incident light. This is known as Rayleigh scattering.
However, a small fraction of the scattered light exhibits a frequency shift due to the exchange of energy between the incident photons and...
373
Raman Spectroscopy Instrumentation: Overview01:26

Raman Spectroscopy Instrumentation: Overview

332
A conventional Raman spectrophotometer includes a laser source, a sample holding system, a wavelength selector, and a detector.
The monochromatic laser source, typically using visible or near-infrared radiation, generates a highly focused beam of light. This light interacts with the molecules of the sample, scattering some of the light. Liquid and gaseous samples are usually tested in ordinary glass capillaries, while solids can be analyzed as powders packed in capillaries or as potassium...
332

您也可能阅读

相关文章

通过共同作者、期刊和引用图与本文相关的文章。

排序
Same author

Antimicrobial Peptide-Loaded Mesoporous Silica Nanoparticles: A pH-Triggered Controlled Release against Biofilms.

ACS omega·2025
Same author

Xylitol Modification of Electrospun Polymer Scaffolds: Impact on Physicochemical and Antibacterial Properties.

Polymers·2025
Same author

Engineering Poly(L-Lactic Acid)/Hydroxyapatite Scaffolds via Melt-Electrowriting: Enhancement of Osteochondral Cell Response in Human Nasal Chondrocytes.

Polymers·2025
Same author

Enhanced Piezoelectricity in Sustainable-By-Design Chitosan Nanocomposite Soft Thin Films for Green Sensors.

ACS nano·2025
Same author

Effects of Post-Curing on Mechanical Strength and Cytotoxicity of Stereolithographic Methacrylate Resins.

Polymers·2025
Same author

Mapping Selenium Nanoparticles Distribution Inside Cells through Confocal Raman Microspectroscopy.

ACS applied materials & interfaces·2025

相关实验视频

Updated: Jun 28, 2025

Identification of Disease-related Spatial Covariance Patterns using Neuroimaging Data
14:27

Identification of Disease-related Spatial Covariance Patterns using Neuroimaging Data

Published on: June 26, 2013

15.6K

拉曼成像的统计方法:主要成分得分映射.

Elia Marin1,2,3,4, Davide Redolfi Bristol1,5,6, Alfredo Rondinella3

  • 1Ceramic Physics Laboratory, Kyoto Institute of Technology, Sakyo-ku, Matsugasaki, 606-8585 Kyoto, Japan. elia-marin@kit.ac.jp.

Analytical methods : advancing methods and applications
|April 17, 2024
PubMed
概括

本研究介绍了分析拉曼成像数据的指导方针,特别是使用低强度光谱的大型数据集. 突出指出,对原始光谱数据的统计分析对于在自动化工具不足时提取最大信息至关重要.

更多相关视频

Resolving Water, Proteins, and Lipids from In Vivo Confocal Raman Spectra of Stratum Corneum through a Chemometric Approach
09:32

Resolving Water, Proteins, and Lipids from In Vivo Confocal Raman Spectra of Stratum Corneum through a Chemometric Approach

Published on: September 26, 2019

7.2K
Multiplex Chemical Imaging Based on Broadband Stimulated Raman Scattering Microscopy
09:57

Multiplex Chemical Imaging Based on Broadband Stimulated Raman Scattering Microscopy

Published on: July 25, 2022

3.9K

相关实验视频

Last Updated: Jun 28, 2025

Identification of Disease-related Spatial Covariance Patterns using Neuroimaging Data
14:27

Identification of Disease-related Spatial Covariance Patterns using Neuroimaging Data

Published on: June 26, 2013

15.6K
Resolving Water, Proteins, and Lipids from In Vivo Confocal Raman Spectra of Stratum Corneum through a Chemometric Approach
09:32

Resolving Water, Proteins, and Lipids from In Vivo Confocal Raman Spectra of Stratum Corneum through a Chemometric Approach

Published on: September 26, 2019

7.2K
Multiplex Chemical Imaging Based on Broadband Stimulated Raman Scattering Microscopy
09:57

Multiplex Chemical Imaging Based on Broadband Stimulated Raman Scattering Microscopy

Published on: July 25, 2022

3.9K

科学领域:

  • 频谱学是一种光谱学.
  • 材料科学 材料科学 材料科学
  • 数据分析 数据分析

背景情况:

  • 拉曼成像产生大量低强度光谱的大型数据集.
  • 自动化软件往往在基线校正和数据适合复杂的光谱数据方面遇到困难.
  • 对拉曼成像数据的有效解释对于科学发现至关重要.

研究的目的:

  • 建立分析大型数据集的拉曼成像结果的指导方针.
  • 调查统计分析对从原始光谱数据中提取信息的有用性.
  • 提高拉曼成像数据解释的效率和深度.

主要方法:

  • 拉曼成像被用来绘制各种样本的地图.
  • 使用自动化工具进行初始数据提取 (强度,信号与噪声比).
  • 主要成分分析 (PCA) 用于模式识别和光谱相似性调查.

主要成果:

  • 对原始光谱数据的统计分析被证明是有效的信息提取.
  • 发现自动化软件不足以进行基线删除和数据调整.
  • 根据主要的拉曼波段确定了代表性的地点,以进行质量评估.

结论:

  • 统计分析是最大限度地从大型拉曼成像数据集中获取信息的强大工具.
  • 结合自动化工具和统计分析的强大方法学增强了拉曼成像数据的解释.
  • 这种方法减少了数据分析所需的努力,从复杂的光谱数据中获得更深入的见解.