具有截断轻链转基因表达单域H链唯一抗体的
Philip A Leighton1, Kathryn Ching, Kevin Reynolds1
1OmniAb, Emeryville, CA.
Journal of immunology (Baltimore, Md. : 1950)
|April 17, 2024
概括
转基因可以产生仅重链抗体 (sdAbs),这些抗体较小,并以独特的方式结合目标. 这一突破扩大了抗体工程对各种应用的潜力.
科学领域:
- 免疫学 免疫学 免疫学
- 分子生物学分子生物学
- 生物技术是生物技术.
背景情况:
- 只有重链抗体 (sdAbs) 在驼和鱼中自然存在,由于它们的体积较小,它们具有独特的抗原结合特性.
- 大多数脊椎动物使用由重链和轻链组成的常规抗体,限制了sdAb格式的发展.
- 在像这样的非传统物种中研究sdAb生产可能会扩大抗体工程的范围.
研究的目的:
- 为了设计能够产生重链单抗体 (sdAbs) 的转基因.
- 评估来自的sdAbs.的发育,表达和功能能力.
- 评估作为产生sdAbs.的新平台的潜力.
主要方法:
- 转基因的产生缺乏轻链V区域,仅保留LC区域用于护送功能.
- 分析B细胞种群,抗体谱系多样性 (IgM) 和同型切换.
- 用各种蛋白质免疫原和SARS-CoV-2免疫接种转基因.
- 通过单个B细胞查和体外功能测定,通过高亲和度单克隆抗体 (mAb) 恢复.
主要成果:
- 转基因产生了可活的B细胞群体,并表达了序列sdAbs,在重链中自发CH1删除.
- 观察到多样化的IgM谱,没有同型转换为IgY或IgA.
- 免疫迅速诱导高,特定的血清标位,高亲和度的mAbs被有效地恢复.
- 在实验室中,来自的sdAbs证明了强大的中和和预防SARS-CoV-2复制.
结论:
- 截断的轻链设计成功实现了sdAb在的发展和表达.
- 转基因代表了一个可行的和有前途的新宿主系统,用于产生功能性sdAbs.
- 来自的sdAbs具有治疗和诊断应用的巨大潜力,包括对抗像SARS-CoV-2这样的病毒病原体.
更多相关视频
00:09Single-cell Screening Method for the Selection and Recovery of Antibodies with Desired Specificities from Enriched Human Memory B Cell Populations
Published on: August 22, 2019
10.7K
09:23Parasite Induced Genetically Driven Autoimmune Chagas Heart Disease in the Chicken Model
Published on: July 29, 2012
13.9K
相关概念视频
01:31Hybridoma Technology
14.7K
Hybridoma technology is used for the large-scale production of monoclonal antibodies. Monoclonal antibodies bind to only a single antigenic determinant or epitope. Such antibodies are used in research, diagnostics, and disease therapy. The hybridoma technology established in 1975 by Georges Köhler and Cesar Milstein was awarded the Nobel Prize in Medicine in 1984 for revolutionizing research and therapy.
Hybridoma Selection
Commonly used fusion techniques — electroporation,...
Hybridoma Selection
Commonly used fusion techniques — electroporation,...
14.7K
01:10Antibody Structure
60.1K
Overview
Antibodies, also known as immunoglobulins (Ig), are essential players of the adaptive immune system. These antigen-binding proteins are produced by B cells and make up 20 percent of the total blood plasma by weight. In mammals, antibodies fall into five different classes, which each elicits a different biological response upon antigen binding.
The Y-Shaped Structure of Antibodies Consists of Four Polypeptide Chains
Antibodies consist of four polypeptide chains: two identical heavy...
Antibodies, also known as immunoglobulins (Ig), are essential players of the adaptive immune system. These antigen-binding proteins are produced by B cells and make up 20 percent of the total blood plasma by weight. In mammals, antibodies fall into five different classes, which each elicits a different biological response upon antigen binding.
The Y-Shaped Structure of Antibodies Consists of Four Polypeptide Chains
Antibodies consist of four polypeptide chains: two identical heavy...
60.1K
01:25Antibody Structure and Classes
898
Antibodies, also known as immunoglobulins, are produced by B cells in response to foreign substances, such as bacteria and viruses. These proteins are critical for recognizing and neutralizing these substances, protecting the body from potential harm.
The basic structure of an antibody consists of four protein chains: two identical heavy chains and two identical light chains. These chains are held together by disulfide bonds and other non-covalent interactions, forming a Y-shaped structure.
The basic structure of an antibody consists of four protein chains: two identical heavy chains and two identical light chains. These chains are held together by disulfide bonds and other non-covalent interactions, forming a Y-shaped structure.
898