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在Arthrobotrys oligospora中响应基的微RNA的表达特征和特征

Ningxing Li¹, Yansen Sun¹, Yucheng Liu²

¹College of Animal Science and Technology, Shihezi University, North Street No.4, Shihezi, 832003, Xinjiang, China.

Archives of microbiology

|April 17, 2024

概括

这项研究在Arthrobotrys oligospora中确定了新型的微RNA (miRNA),它们对素有反应,通过转录后控制调节细胞增殖和酶表达等基本过程.

科学领域:

菌类学菌类学是指菌类学.
分子生物学分子生物学
基因组学就是基因组学.

背景情况:

细胞外蛋白酶,如来自Arthrobotrys oligospora的基因酶,是线虫感染的关键.
在A. oligospora中,微RNAs (miRNAs) 的转录后基因调节是鲜为人知的.

研究的目的:

分析A. oligospora.中的素反应性miRNA表达特征.
识别和描述差异表达的miRNAs (DEmiRNAs) 和它们的目标基因.

主要方法:

转录基因组测序 (RNA-seq) 用于描述miRNA的表达.
对miRNA目标的生物信息预测 (miRanda,Targetscan).
双露西法酶记者测定用于验证miRNA-目标相互作用.

主要成果:

鉴定了85种新的miRNA,其中25种DEmiRNA对基的反应.
在生物降解,细胞外成分和细胞循环途径方面,DEmiRNA的点更加丰富.
已确认miR_70的向酸酶AOL_s00004g379,对于酸水解至关重要.

结论:

基受敏的miRNAs调节了A. oligospora的细胞增殖,细胞生长和基酶的产生.
这项研究为A. oligospora的miRNA介导的转录后基因调节提供了新的见解.

关键词:

这种植物是Arthrobotrys oligospora.基反应的小RNAs.基因酸酶的使用方法对基因表达的调节

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相关概念视频

MicroRNAs

MicroRNAs

MicroRNA (miRNA) are short, regulatory RNA transcribed from introns (non-coding regions of a gene) or intergenic regions (stretches of DNA present between genes). Several processing steps are required to form biologically active, mature miRNA. The initial transcript, called primary miRNA (pri-mRNA), base-pairs with itself, forming a stem-loop structure. Within the nucleus, an endonuclease enzyme, called Drosha, shortens the stem-loop structure into hairpin-shaped pre-miRNA. After the pre-miRNA...