相关实验视频

Updated: Jun 28, 2025

Detection of Axonally Localized mRNAs in Brain Sections Using High-Resolution In Situ Hybridization
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Detection of Axonally Localized mRNAs in Brain Sections Using High-Resolution In Situ Hybridization

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在现场混合化大脑切片

Tomohiro Kanayama1, Hiroyuki Tomita2, Akira Hara2

  • 1Department of Tumor Pathology, Gifu University Graduate School of Medicine, Gifu, Japan. kanayama.tomohiro.u7@f.gifu-u.ac.jp.

Methods in molecular biology (Clifton, N.J.)
|April 17, 2024
PubMed
概括

在现场杂交 (ISH) 使用mRNA检测大脑切片中的基因表达. 这种方法现在适用于用于脑瘤研究的甲固定嵌入样本.

关键词:
大脑的切片是大脑的一部分.嵌入式法固定的法 (FFPE) 的形式素固定的法在现场混合化.鼠标 鼠标是一个鼠标.

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相关概念视频

In-situ Hybridization02:31

In-situ Hybridization

In situ hybridization (ISH) is a technique used to detect and localize specific DNA or RNA molecules in cells, tissue, or tissue sections using a labeled probe. The technique was first used in 1969 for the investigation of nucleic acids. It is currently an essential tool in scientific research and clinical settings, especially for diagnostic purposes.
Types of probes and labels
A probe is a complementary strand of DNA or RNA that binds to corresponding nucleotide sequences in a cell. Many...
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