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相关概念视频

Preparation of Samples for Electron Microscopy01:20

Preparation of Samples for Electron Microscopy

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To be visualized by an electron microscope, either transmission or scanning, biological samples need to be fixed (stabilized) so the electron beam does not destroy them and dried thoroughly (desiccated/dehydrated) so the vacuum does not affect them. Fixation needs to be done as quickly as possible because the sample properties will start changing as soon as it is removed from its natural environment. For example, in a tissue sample, the oxygen levels begin decreasing, causing an altered...
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相关实验视频

Updated: Jun 28, 2025

Preparation of Mouse Brain Tissue for Immunoelectron Microscopy
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用于扫描电子显微镜的小鼠大脑组织准备.

Kosuke Mori1, Chihiro Takada2, Hideshi Okada2

  • 1Departments of Tumor Pathology, Gifu University Graduate School of Medicine, Gifu, Japan.

Methods in molecular biology (Clifton, N.J.)
|April 17, 2024
PubMed
概括

扫描电子显微镜 (SEM) 提供了详细的3D表面成像. 一种新的固定和冷裂解方法使得在像老鼠大脑这样的富含水的样本中能够进行高分辨率的超结构观测.

关键词:
冰裂解压器是什么?在冷干燥过程中进行冷干燥.perfusion 固定方式的固定方式.扫描电子显微镜扫描电子显微镜

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Specimen Preparation, Imaging, and Analysis Protocols for Knife-edge Scanning Microscopy
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Specimen Preparation, Imaging, and Analysis Protocols for Knife-edge Scanning Microscopy

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Micron-scale Resolution Optical Tomography of Entire Mouse Brains with Confocal Light Sheet Microscopy
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Micron-scale Resolution Optical Tomography of Entire Mouse Brains with Confocal Light Sheet Microscopy

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相关实验视频

Last Updated: Jun 28, 2025

Preparation of Mouse Brain Tissue for Immunoelectron Microscopy
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Specimen Preparation, Imaging, and Analysis Protocols for Knife-edge Scanning Microscopy
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科学领域:

  • 显微镜的使用方法
  • 生物成像技术 生物成像技术
  • 神经科学是一个神经科学.

背景情况:

  • 扫描电子显微镜 (SEM) 对于观测高分辨率和大视距深度的表面地形至关重要.
  • 传统的SEM方法在保护水合生物组织的超结构方面面临挑战.
  • 裂提供了一种可视化内部结构的途径,但需要优化样本准备.

研究的目的:

  • 为小鼠大脑样本提供一个优化的固定和裂协议.
  • 通过使用SEM. 在水合生物标本中实现超结构的高分辨率成像.
  • 用SEM克服观察微妙生物样本的局限性.

主要方法:

  • 样品制备,包括化学固定小鼠大脑组织.
  • 固定组织的冷破碎以暴露内部结构.
  • 骨折后处理和喷雾涂层用于SEM观察.
  • 使用扫描电子显微镜 (SEM) 进行高分辨率成像.

主要成果:

  • 在固定和冷裂解后,在小鼠大脑组织中成功保存了细微的超结构细节.
  • 细胞和细胞外成分的三维形态的演示.
  • 截面的高分辨率成像,揭示组织结构.

结论:

  • 描述的固定和裂方法对于SEM分析小鼠大脑超结构是有效的.
  • 这种技术显著提高了研究纳米级水合生物样本的能力.
  • 该协议促进了组织形态和细胞组织的详细调查.