将蛋白酶活性与食用的杀菌作用联系起来
Adriane Toledo da Silva1, Debora Castro de Souza1, Stefany Amorim de Souza1
1Departamento de Química, Laboratório de Biotecnologia e Bioquímica Aplicada, Universidade Federal de Lavras, Lavras, Brazil.
World journal of microbiology & biotechnology
|April 17, 2024
概括
可食用,特别是Pleurotus物种,对线虫有显著的杀菌活性. 这些真菌产生的蛋白酶是它们在可持续害虫管理中的生物控制潜力的关键.
科学领域:
- 农业科学 农业科学
- 菌类学 菌类学是指菌类学.
- 生物技术是生物技术.
背景情况:
- 使用食用进行生物控制,为害虫管理提供了一种可持续的方法.
- 虽然已知真菌毒素是杀虫剂,但Pleurotus物种蛋白酶在线虫控制中的作用尚未得到充分研究.
研究的目的:
- 为了研究不同种类的Pleurotus中的蛋白酶的杀菌活性.
- 为了将蛋白酶的产生与食用对线虫的疗效相关联.
主要方法:
- 对Panagrellus sp.的运动性测定 这些研究是使用Pleurotus sp.,P. ostreatus (SB),P. ostreatus (Pearl) 和P. djamor.的无细胞提取物进行的.
- 蛋白酶活性在各种pH值和发酵时间 (45天和75天) 测量.
主要成果:
- 所有测试的食用显著减少了超过82%的线虫养活动.
- 没有细胞的原始提取物表现出显著的尼马杀效应.
- 在45天后,P. djamor显示了73%的减少;Pleurotus sp. 在75天的时间里,P. ostreatus (Pearl) 和P. ostreatus (Pearl) 分别减少了64%和62%.
结论:
- 蛋白酶活性与Pleurotus物种的杀性作用直接相关.
- 这些发现突显了Pleurotus蛋白酶在生物控制线虫方面的潜力.
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