在RNA样本准备过程中,循环N6 - - 氨基胺氨酸的化
Larissa Bessler1, Jason Sirleaf2, Christopher J Kampf2
1Institute of Pharmaceutical and Biomedical Sciences, Johannes Gutenberg University Mainz, Staudingerweg 5, 55128, Mainz, Germany.
ChemMedChem
|April 17, 2024
概括
研究人员发现了一种模仿新RNA修饰的工件. 这种N6-threonylcarbamoyladenine (t6A) 的糖醇体在体外形成,强调在识别RNA修饰时需要谨慎.
科学领域:
- 生物化学 生物化学
- 分子生物学分子生物学
- 分析化学 分析化学
背景情况:
- 由于它们的生物学作用和治疗潜力,对RNA修饰的兴趣越来越大.
- 确定修改后核糖核酸的既定方法包括LC-MS/MS和稳定同位素标记.
- 在准确识别和量化RNA修饰方面存在挑战.
研究的目的:
- 为了从结构上阐明一个新发现的核酸结构.
- 调查这个结构的起源和形成机制.
- 评估对RNA修饰发现和量化的影响.
主要方法:
- 液体染色学与核酸水平的双重质谱学 (LC-MS/MS) 相结合.
- 稳定同位素标记实验.
- 结构阐明和化学分析.
主要成果:
- 一个最初被认为是真正的RNA修饰的新型结构被确定.
- 这种结构被确定为一个体外人工物:N6-threonylcarbamoyladenine (t6A) 的糖醇.
- 该工件是由N6-((cis-4-hydroxy-2-methyl) but-2-enyl) carbamoyladenine (ct6A) 在性条件下的含糖醇缓冲体中的RNA水解过程中形成的.
- 文物经历了分子内转化.
结论:
- 该研究发现了一种常见的工件,可以被误认为是一种新型RNA修饰.
- 结果强调了在发现和结构验证新RNA修饰时需要更加谨慎.
- 化学不稳定的修饰,如ct6A,在样本准备过程中需要小心处理,以避免误识和不准确的量化.
关键词:
副本转录组 (epitranscriptome) 是一个副本转录组 (epitranscriptome).水解工艺品的工件质谱测量质谱测量质谱测量质量测量质谱测量质量测量质量测量质量测量质量测量质量测量质量测量质量测量质量测量质量测量质量测量质量测量质量测量质量测量质量测量质量测量质量测量质量测量质量测量质量测量核酸分析的核酸分析这是一个tRNARNA.更多相关视频
11:37Protocol for the Solid-phase Synthesis of Oligomers of RNA Containing a 2'-O-thiophenylmethyl Modification and Characterization via Circular Dichroism
Published on: July 28, 2017
19.0K
08:49Improving Small RNA-seq: Less Bias and Better Detection of 2'-O-Methyl RNAs
Published on: September 16, 2019
7.6K
相关概念视频
01:23RNA Structure
Overview
The basic structure of RNA consists of a five-carbon sugar and one of four nitrogenous bases. Although most RNA is single-stranded, it can form complex secondary and tertiary structures. Such structures play essential roles in the regulation of transcription and translation.
Different Types of RNA Have the Same Basic Structure
There are three main types of ribonucleic acid (RNA): messenger RNA (mRNA), transfer RNA (tRNA), and ribosomal RNA (rRNA). All three RNA types consist of a...
The basic structure of RNA consists of a five-carbon sugar and one of four nitrogenous bases. Although most RNA is single-stranded, it can form complex secondary and tertiary structures. Such structures play essential roles in the regulation of transcription and translation.
Different Types of RNA Have the Same Basic Structure
There are three main types of ribonucleic acid (RNA): messenger RNA (mRNA), transfer RNA (tRNA), and ribosomal RNA (rRNA). All three RNA types consist of a...
02:23RNA Editing
RNA editing is a post-transcriptional modification where a precursor mRNA (pre-mRNA) nucleotide sequence is changed by base insertion, deletion, or modification. The extent of RNA editing varies from a few hundred bases, in mitochondrial DNA of trypanosomes, to a just single base, in nuclear genes of mammals. Even a single base change in the pre-mRNA can convert a codon for one amino acid into the codon for another amino acid or a stop codon. This type of re-coding can significantly affect the...
01:19RNA Structure
The basic structure of RNA consists of a string of ribonucleotides attached by phosphodiester bonds. Although most RNA is single-stranded, it can form complex secondary and tertiary structures. Such structures play essential roles in the regulation of transcription and translation.
Different Types of RNA Have the Same Basic Structure
There are three main types of ribonucleic acid (RNA) involved in protein synthesis: messenger RNA (mRNA), transfer RNA (tRNA), and ribosomal RNA (rRNA). All three...
Different Types of RNA Have the Same Basic Structure
There are three main types of ribonucleic acid (RNA) involved in protein synthesis: messenger RNA (mRNA), transfer RNA (tRNA), and ribosomal RNA (rRNA). All three...
01:35Pre-mRNA Processing: Modification of pre-mRNA Ends
In eukaryotic cells, transcripts made by RNA polymerase are modified and processed before exiting the nucleus. Unprocessed RNA is called precursor mRNA or pre-mRNA to distinguish it from mature mRNA.
Once about 20-40 ribonucleotides have been joined together by RNA polymerase, a group of enzymes adds a cap to the 5' end of the growing transcript. In this process, a 5' phosphate is replaced by modified guanosine that has a methyl group attached (7-methyl guanosine). This 5' cap helps the cell...
Once about 20-40 ribonucleotides have been joined together by RNA polymerase, a group of enzymes adds a cap to the 5' end of the growing transcript. In this process, a 5' phosphate is replaced by modified guanosine that has a methyl group attached (7-methyl guanosine). This 5' cap helps the cell...