黑色素减轻了内分泌网膜的压力,通过调节老年产母的mTOR通路来改善卵巢功能
Er-Ying Hao1, Xue-Lu Liu1, Li-Yun Chang2
1College of Animal Science and Technology, Hebei Agricultural University, Baoding, Hebei 071001, China.
Poultry science
|April 17, 2024
概括
黑素 (MEL) 通过激活mTOR信号和减少内分泌网膜应激 (ERS) 来增强老的产卵能力,促进毛囊发育和改善生殖性能.
科学领域:
- 生殖生物学 生殖生物学
- 分子内分泌学分子内分泌学
- 细胞应激反应的细胞应激反应
背景情况:
- 颗粒细胞亡有助于卵泡缩和减少老的卵子产量.
- 细胞内膜网膜应激 (ERS) 是一种新兴的途径,涉及到卵泡缩,可能由mTOR调节.
- 众所周知,黑素 (MEL) 支持毛囊发育,但其确切的机制尚不清楚.
研究的目的:
- 为了研究黑激素,内分泌网膜应激和老母mTOR信号通路之间的关系.
- 阐明 Melatonin 影响毛囊发育和产卵率的分子机制.
主要方法:
- 在年龄较大的母中进行内注射的黑激素.
- 测量血激素度 (MEL,E2,LH,FSH) 和关键基因的mRNA表达 (mTOR通路和ERS标志物).
- 在实验室中用黑素对老化中的颗粒细胞进行治疗,然后进行RNA测序和RT-PCR分析.
主要成果:
- 黑色素治疗逆转了老母的产卵率,卵巢卵泡数量和激素水平的下降.
- 黑色素降低了ERS标记物 (Grp78,CHOP,Caspase-3) 和增加了mTOR信号基因表达.
- RNA-seq分析确定了参与细胞生长,细胞亡,mTOR信号传递和ER压力的差异表达基因.
结论:
- 黑色素促进毛囊发育,增强老母的产卵能力.
- 黑色素通过激活mTOR信号通路并减轻内分泌网膜压力来发挥其作用.
- 这项研究为提高母生产率和理解卵泡选择机制提供了理论基础.
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