Nrf2/ARE通路作为一个潜在的目标,以改善粒状细胞中阿特拉诱导的内分泌干扰
Xiao-Wei Li1, Kai Guo2, Chi-Chiu Wang3
1College of Veterinary Medicine, Northeast Agricultural University, Harbin, 150030, China.
Poultry science
|April 17, 2024
概括
阿特拉辛 (ATR) 和其代谢物DACT会损害鸟类颗粒细胞,破坏类固醇生产和生殖功能. 准Nrf2/ARE通路可能会减轻这些毒性影响.
科学领域:
- 环境毒理学环境毒理学
- 生殖生物学 生殖生物学
- 细胞生物学 细胞生物学
背景情况:
- 阿特拉 (ATR) 是一种广泛使用的除草剂,已知具有内分泌干扰作用.
- 氨基酸 (DACT) 是一种主要的ATR代谢物,对有机体产生影响.
- 颗粒状细胞 (GCs) 对鸟类繁殖至关重要,但ATR对鸟类GCs的毒性尚不清楚.
研究的目的:
- 为了研究阿特拉辛 (ATR) 和其代谢物大米诺酸 (DACT) 对鸟类颗粒细胞 (GCs) 的毒性作用.
- 阐明ATR诱导的鸟类生殖障碍背后的机制.
主要方法:
- Quail GCs 被暴露于不同度的ATR和DACT.
- 评估了细胞活力,线粒体结构和膜潜力.
- 分析了类固醇生成基因表达和激素分泌 (E2,P).
- 研究了细胞亡和Nrf2/ARE通路.
主要成果:
- ATR显著降低了GC的活力,并破坏了线粒体完整性.
- 暴露于ATR改变了类固醇合成途径,增加了E2和P分泌.
- 在GC中,ATR和DACT都诱导了亡.
- 确定了Nrf2/ARE通路作为减轻ATR毒性的潜在目标.
结论:
- 阿特拉辛及其代谢物DACT对鸟类颗粒细胞功能和生殖健康构成重大威胁.
- 线粒体功能障碍和亡是ATR毒性的关键机制.
- Nrf2/ARE 途径为抵消鸟类ATR诱导的内分泌干扰提供了一个有希望的治疗标.
更多相关视频
05:28Monitoring On-Target Signaling Responses in Larval Zebrafish - Z-REX Unmasks Precise Mechanisms of Electrophilic Drugs and Metabolites
Published on: June 2, 2023
804
10:51Reverse Yeast Two-hybrid System to Identify Mammalian Nuclear Receptor Residues that Interact with Ligands and/or Antagonists
Published on: November 15, 2013
12.8K
相关概念视频
01:31Transducer Mechanism: Nuclear Receptors
1.3K
Nuclear receptors, or NRs, are unique transcription factors that regulate gene transcription and affect the cellular pathways involved in reproduction, development, or metabolism. Their ability to be stimulated by small lipophilic ligands and control vital cellular processes makes them ideal drug targets. Nearly 10-15% of currently prescribed drugs target these receptors.
About 48 different soluble family members of nuclear receptors are identified that can be divided into two main classes:
About 48 different soluble family members of nuclear receptors are identified that can be divided into two main classes:
1.3K
01:22Target Cell Response to Hormones
3.0K
Hormones intricately bind to receptors on the surface or within target cells, initiating a cascade of cellular responses.
Notably, the cellular response can be regulated by altering the number of receptors expressed in the cell. For example, prolonged exposure to elevated hormone levels results in a gradual decline or down-regulation in the number of receptors for that specific hormone on the cell surface. Conversely, in response to low hormone levels, cells may use up-regulation, producing an...
Notably, the cellular response can be regulated by altering the number of receptors expressed in the cell. For example, prolonged exposure to elevated hormone levels results in a gradual decline or down-regulation in the number of receptors for that specific hormone on the cell surface. Conversely, in response to low hormone levels, cells may use up-regulation, producing an...
3.0K
01:25Cell Signaling in Plants
5.6K
Plant cells communicate to coordinate their cycle of growth, flowering and fruiting, and activities in roots, shoots, and leaves in response to the changing environmental conditions. Plant signaling is distinct from animal signaling. Plants primarily utilize enzyme-linked receptors, whereas the largest class of cell-surface receptors in animals are G-protein coupled receptors (GPCRs). Unlike animals, receptor tyrosine kinases are rare in plants. Instead, plants have a diverse class of...
5.6K