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Updated: Jun 28, 2025

Identification of Circular RNAs using RNA Sequencing

Identification of Circular RNAs using RNA Sequencing

Published on: November 14, 2019

通过从微阵列中选circRNAs介导的ceRNA网络的构建,并识别了髓灰质炎重症的新生物标志物

Xiaotong Kong¹, Tao Wu², Hanlu Cai¹

¹Department of Neurology, The Second Affiliated Hospital, Harbin Medical University, Harbin, Heilongjiang Province, China.

|April 17, 2024

概括

循环RNAs (circRNAs) 与自身免疫性疾病有关. 这项研究通过circFRMD4/miR-145-5p轴确定circFRMD4是通过circFRMD4/miR-145-5p轴参与肌痛性硬化症 (MG) 发病的一个关键circRNA,表明其作为生物标志物的潜力.

科学领域:

分子生物学分子生物学
免疫学免疫学免疫学
遗传学遗传学是一个

背景情况:

循环RNAs (circRNAs) 越来越多地被认为在自身免疫性疾病中的作用.
了解涉及到Myasthenia Gravis (MG) 的特定circRNA对于开发新的诊断和治疗策略至关重要.

研究的目的:

为了识别MG中的关键circRNAs作为竞争的内源RNAs (ceRNAs).
探索circRNAs作为MG生物标志物的潜力.
阐明在MG病变发生过程中发现的circRNAs的调节机制.

主要方法:

与对照组相比,CircRNA微阵列分析以确定MG患者的差异表达circRNA (DECs).
使用随机步行与重启 (RWR) 算法预测和选目标microRNAs (miRNAs).
构建一个circRNA-miRNA-mRNA (CMMC) 调控网络以识别枢纽分子.
使用RT-PCR验证枢纽环RNA表达.
通过光酶试验和CCK-8试验对circFRMD4海绵型miR-145-5p在调节细胞增殖中的功能验证.

主要成果:

在MG患者中观察到circFRMD4和circPIGB的上调,以及circNUP214的显著下调.
证实circFRMD4可以菌miR-145-5p,从而调节Jurkat细胞的增殖.
鉴定出circFRMD4/miR-145-5p轴是MG发展中的关键调节途径.

结论:

circFRMD4,circPIGB和circNUP214显示出可能成为阳性乙胆受体 (AchR+) MG 的新生物标志物.
circFRMD4通过与miR-145-5p的相互作用,在AchR+ MG的发病过程中发挥着重要作用.

关键词:

生物标志物生物标志物循环RNA 是一个循环RNA.骨髓灰质炎 (Myasthenia gravis) 是一个严重的疾病.ceRNARNA是什么意思

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