相关实验视频

Updated: Jun 28, 2025

Reliably Engineering and Controlling Stable Optogenetic Gene Circuits in Mammalian Cells

Reliably Engineering and Controlling Stable Optogenetic Gene Circuits in Mammalian Cells

Published on: July 6, 2021

在哺乳动物系统中设计一种合成基因电路,用于高性能诱导表达

Giuliano De Carluccio^1,2,3, Virginia Fusco^1,2, Diego di Bernardo^4,5

¹Telethon Institute of Genetics and Medicine, Naples, Italy.

Nature communications

|April 17, 2024

概括

我们开发了CASwitch,一种合成基因电路,可以减少不必要的基因表达 (泄漏),同时保持高输出. 这种新的系统增强了对生物传感器和基因治疗等应用的控制.

科学领域:

合成生物学合成生物学
分子和细胞生物学分子和细胞生物学
生物技术是生物技术.

背景情况:

用小分子控制基因表达是至关重要的,但通常涉及低背景 (泄漏) 和高诱导表达之间的权衡.
现有的诱导系统经常在最小化非诱导表达和最大化诱导表达之间做出妥协.

研究的目的:

开发一种新的合成基因电路,CASwitch,缓解泄漏,同时保持高诱导基因表达,而不改变转录因子或促进体.
应用定量合成生物学原理来设计一个强大的哺乳动物基因电路.

主要方法:

设计了CASwitch电路,结合了连贯输入前传循环 (CFFL) 和相互抑制 (MI) 网络模式.
整合了CRISPR-Cas内核酶CasRx与Tet-On3G可诱导基因系统.
利用数学建模和实验验证用于电路设计和性能评估.

主要成果:

CASwitch系统在缓解泄漏和实现显著的诱导表达方面表现出高性能.
成功应用CASwitch来增强全细胞生物传感器的灵敏度.
在控制有毒基因表达和启用诱导性产生腺相关病毒 (AAV) 载体方面展示了实用性.

结论:

CASwitch提供了一种强大的新工具,用于精确控制哺乳动物系统中的基因表达.
合成基因电路显示出各种生物技术应用的巨大潜力,包括基因治疗和生物传感.
这项工作强调了将网络图案和CRISPR技术结合起来,用于先进的合成生物学设计的有效性.

更多相关视频

Inducible T7 RNA Polymerase-mediated Multigene Expression System, pMGX

Inducible T7 RNA Polymerase-mediated Multigene Expression System, pMGX

Published on: June 27, 2017

Immunometabolic Circuits in Infection for Advancing Host Directed Therapies

Immunometabolic Circuits in Infection for Advancing Host Directed Therapies

Published on: September 13, 2024

相关概念视频

Reporter Genes

Reporter Genes

Reporter genes are a type of protein-coding gene that are often tagged to a gene of interest. Once inside a target cell, reporter genes usually produce visually identifiable characteristics like fluorescence and luminescence when expressed along with the gene of interest. Thus, reporter genes “report” the presence or absence of genes of interest in an organism, determine the gene expression pattern, or track the physical location of a DNA segment or protein in the cell.

Synthetic Biology

Synthetic Biology

Synthetic biology is an interdisciplinary science that involves using principles from disciplines such as engineering, molecular biology, cell biology, and systems biology. It involves remodeling existing organisms from nature or constructing completely new synthetic organisms for applications such as protein or enzyme production, bioremediation, value-added macromolecule production, and the addition of desirable traits to crops, to name a few.
Golden rice
Golden rice is a genetically modified...

Transgenic Organisms

Transgenic Organisms

In-vitro Mutagenesis

In-vitro Mutagenesis

To learn more about the function of a gene, researchers can observe what happens when the gene is inactivated or “knocked out,” by creating genetically engineered knockout animals. Knockout mice have been particularly useful as models for human diseases such as cancer, Parkinson’s disease, and diabetes.

The Central Dogma

The Central Dogma

The central dogma explains the flow of genetic information from DNA nucleotides to the amino acid sequence of proteins.
RNA is the Missing Link Between DNA and Proteins
In the early 1900s, scientists discovered that DNA stores all the information needed for cellular functions and that proteins perform most of these functions. However, the mechanisms of converting genetic information into functional proteins remained unknown for many years. Initially, it was believed that a single gene is...