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相关概念视频

Viral Recombination00:57

Viral Recombination

23.4K
Cells are sometimes infected by more than one virus at once. When two viruses disassemble to expose their genomes for replication in the same cell, similar regions of their genomes can pair together and exchange sequences in a process called recombination. Alternatively, viruses with segmented genomes can swap segments in a process called reassortment.
23.4K
Viral Mutations00:36

Viral Mutations

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A mutation is a change in the sequence of bases of DNA or RNA in a genome. Some mutations occur during replication of the genome due to errors made by the polymerase enzymes that replicate DNA or RNA. Unlike DNA polymerase, RNA polymerase is prone to errors because it is not capable of “proofreading” its work. Viruses with RNA-based genomes, like HIV, therefore accrue mutations faster than viruses with DNA-based genomes. Because mutation and recombination provide the raw material...
32.3K
Conservative Site-specific Recombination and Phase Variation02:53

Conservative Site-specific Recombination and Phase Variation

6.0K
Because the DNA segments are cut and reorganized in a direction-specific manner, site-specific recombination has emerged as an efficient genetic engineering technique. Flippase and Cyclization recombinases or Flp and Cre, respectively, are two members of the tyrosine recombinase family derived from bacteriophages, that are used to mediate site-specific DNA insertions, deletions, and targeted expression of proteins in mammalian cell lines.
The recognition sites for Cre recombinase called LoxP...
6.0K
Homologous Recombination02:31

Homologous Recombination

50.5K
The basic reaction of homologous recombination (HR) involves two chromatids that contain DNA sequences sharing a significant stretch of identity. One of these sequences uses a strand from another as a template to synthesize DNA in an enzyme-catalyzed reaction. The final product is a novel amalgamation of the two substrates. To ensure an accurate recombination of sequences, HR is restricted to the S and G2 phases of the cell cycle. At these stages, the DNA has been replicated already and the...
50.5K

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相关实验视频

Updated: Jun 28, 2025

Isolation of Fidelity Variants of RNA Viruses and Characterization of Virus Mutation Frequency
18:10

Isolation of Fidelity Variants of RNA Viruses and Characterization of Virus Mutation Frequency

Published on: June 16, 2011

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在病毒基因组中数据驱动的重组检测.

Tommaso Alfonsi1, Anna Bernasconi2, Matteo Chiara3

  • 1Department of Electronics, Information, and Bioengineering, Politecnico di Milano, Via Ponzio 34/5, 20133, Milan, Italy.

Nature communications
|April 17, 2024
PubMed
概括
此摘要是机器生成的。

RecombinHunt准确地识别了复合病毒基因组,包括SARS-CoV-2和麻疹,证实了专家的分析. 这种数据驱动的方法为检测病毒重组事件提供了高准确性和速度.

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Rapid, Seamless Generation of Recombinant Poxviruses using Host Range and Visual Selection
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Rapid, Seamless Generation of Recombinant Poxviruses using Host Range and Visual Selection

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Amplification, Next-generation Sequencing, and Genomic DNA Mapping of Retroviral Integration Sites
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Amplification, Next-generation Sequencing, and Genomic DNA Mapping of Retroviral Integration Sites

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相关实验视频

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Isolation of Fidelity Variants of RNA Viruses and Characterization of Virus Mutation Frequency
18:10

Isolation of Fidelity Variants of RNA Viruses and Characterization of Virus Mutation Frequency

Published on: June 16, 2011

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Rapid, Seamless Generation of Recombinant Poxviruses using Host Range and Visual Selection
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Rapid, Seamless Generation of Recombinant Poxviruses using Host Range and Visual Selection

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Amplification, Next-generation Sequencing, and Genomic DNA Mapping of Retroviral Integration Sites
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Amplification, Next-generation Sequencing, and Genomic DNA Mapping of Retroviral Integration Sites

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科学领域:

  • 病毒学 病毒学
  • 基因组学就是基因组学.
  • 生物信息学是一种生物信息学.

背景情况:

  • 病毒重组对于进化和适应至关重要.
  • 超过90个SARS-CoV-2血统被认为是复合的.
  • 现有的检测方法缺乏专家手动分析的准确性.

研究的目的:

  • 推出RecombinHunt,这是一种用于识别重组病毒基因组的新型数据驱动方法.
  • 准确检测SARS-CoV-2和其他病毒复合物,具有一个或两个断点.
  • 为病毒重组分析提供可靠和有效的工具.

主要方法:

  • 开发了一种原始的数据驱动计算方法,命名为RecombinHunt.
  • 应用RecombinHunt用于识别复合的SARS-CoV-2基因组和血统.
  • 验证了RecombinHunt的性能与手动专家分析和其他最先进的方法相比.
  • 测试了RecombinHunt对来自疫的复合病毒基因组的测试.

主要成果:

  • RecombinHunt准确地识别了复合的SARS-CoV-2基因组和高特异性和灵敏性的血统.
  • 该方法与手动专家分析具有很高的一致性.
  • 与现有的最先进的重组检测工具相比,RecombinHunt的表现良好.
  • 该工具成功识别了复合病毒病毒基因组.

结论:

  • RecombinHunt是一种强大而准确的方法来识别病毒重组.
  • 与手动分析相比,这种方法可以缩短周转时间.
  • RecombinHunt的有效性表明它适用于任何流行病或流行病病毒.