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相关概念视频

Introduction to Fibroblasts01:09

Introduction to Fibroblasts

3.1K
Rudolph Virchow discovered spindle-shaped cells called fibroblasts in 1858. Inactive fibroblasts, called fibrocytes, become activated by various stimuli, such as growth factors and inflammatory cytokines. Activated fibroblasts play a crucial role in wound healing, inflammation, formation of new blood vessels, and cancer progression. Uncontrolled activation of fibroblasts results in fibrosis, the excess deposition of fibrous tissue, which can lead to scarring and affect normal organs. This...
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Somatic to iPS Cell Reprogramming01:29

Somatic to iPS Cell Reprogramming

2.2K
Reprogramming alters the gene expression in somatic cells, transforming them into induced pluripotent stem (iPS) cells over several generations. Scientists can reprogram cells by introducing genes for four transcription factors—Oct4, Sox2, Klf4, and c-Myc (OSKM) by viral or non-viral methods. These factors are also known as Yamanaka factors after Shinya Yamanaka, who first generated iPS cells using mouse skin cells. Yamanaka was awarded the Nobel Prize in Physiology or Medicine in 2012...
2.2K
Induced Pluripotent Stem Cells01:13

Induced Pluripotent Stem Cells

22.7K
Stem cells are undifferentiated cells that divide and produce different types of cells. Ordinarily, cells that have differentiated into a specific cell type are post-mitotic—that is, they no longer divide. However, scientists have found a way to reprogram these mature cells so that they “de-differentiate” and return to an unspecialized, proliferative state. These cells are also pluripotent like embryonic stem cells—able to produce all cell types—and are therefore...
22.7K
Methods of Nuclear Reprogramming01:24

Methods of Nuclear Reprogramming

1.8K
Nuclear reprogramming is a process of transforming one cell type into an unrelated cell type by epigenetic changes that alter the cell’s original gene expression pattern. Such epigenetic changes force cells to express a different set of genes, which play a significant role in inducing transformation into other cell types. Nuclear reprogramming offers applications in reproductive cloning for livestock propagation and regenerative medicine — developing patient-specific cells for...
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相关实验视频

Updated: Jun 28, 2025

Isolation of Adult Human Dermal Fibroblasts from Abdominal Skin and Generation of Induced Pluripotent Stem Cells Using a Non-Integrating Method
10:52

Isolation of Adult Human Dermal Fibroblasts from Abdominal Skin and Generation of Induced Pluripotent Stem Cells Using a Non-Integrating Method

Published on: January 19, 2020

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将纤维细胞重编程为人类iBlastoids.

Jia Ping Tan1,2,3, Xiaodong Liu4,5,6,7, Jose M Polo8,9,10,11,12

  • 1Department of Anatomy and Developmental Biology, Monash University, Clayton, Victoria, Australia.

Nature protocols
|April 17, 2024
PubMed
概括
此摘要是机器生成的。

研究人员开发了诱导的芽细胞体 (iBlastoids),一种体外人类芽细胞体模型,使用体质重编程. 与目前的方法相比,这种新模型为研究早期人类发展提供了一个更复杂的系统.

更多相关视频

RNA-based Reprogramming of Human Primary Fibroblasts into Induced Pluripotent Stem Cells
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RNA-based Reprogramming of Human Primary Fibroblasts into Induced Pluripotent Stem Cells

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Derivation of Adult Human Fibroblasts and their Direct Conversion into Expandable Neural Progenitor Cells
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Derivation of Adult Human Fibroblasts and their Direct Conversion into Expandable Neural Progenitor Cells

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相关实验视频

Last Updated: Jun 28, 2025

Isolation of Adult Human Dermal Fibroblasts from Abdominal Skin and Generation of Induced Pluripotent Stem Cells Using a Non-Integrating Method
10:52

Isolation of Adult Human Dermal Fibroblasts from Abdominal Skin and Generation of Induced Pluripotent Stem Cells Using a Non-Integrating Method

Published on: January 19, 2020

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RNA-based Reprogramming of Human Primary Fibroblasts into Induced Pluripotent Stem Cells
11:38

RNA-based Reprogramming of Human Primary Fibroblasts into Induced Pluripotent Stem Cells

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Derivation of Adult Human Fibroblasts and their Direct Conversion into Expandable Neural Progenitor Cells
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Derivation of Adult Human Fibroblasts and their Direct Conversion into Expandable Neural Progenitor Cells

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科学领域:

  • 发展生物学 发展生物学
  • 干细胞生物学 干细胞生物学
  • 生殖医学 生殖医学

背景情况:

  • 目前用于早期人类胚胎发生的模型,包括捐赠的胚胎细胞和简化的干细胞培养,在捕捉发育复杂性方面存在局限性.
  • 多能和热囊细胞干细胞具有价值,但缺乏早期胚胎发育的时空和分子动力学.

研究的目的:

  • 描述一种用于产生诱导的布拉斯托因子 (iBlastoids) 的协议,这是人体胚胎囊的体外集成模型.
  • 为解读人类胚胎发生提供一种替代性和更复杂的体外模型.

主要方法:

  • 人体皮肤纤维细胞的体质重编程,以产生重编程中间体.
  • 随后从这些中间体中生成iBlastoids,这个过程大约需要27天.
  • 对iBlastoids的表征和功能测定进行讨论.

主要成果:

  • 成功生成了iBlastoids,一个模仿人类胚胎囊的体外模型.
  • 提供了详细的协议,需要大约一年的细胞培养和重编程测试经验.
  • iBlastoids为研究早期人类发展提供了一个替代工具.

结论:

  • 诱导的Blastoids (iBlastoids) 提供了一个新的,更复杂的体外模型来研究早期人类胚胎发生.
  • 该协议促进科学界对人类发展的探索.
  • 在研究中,iBlastoids 作为传统胚胎囊模型的有价值的替代品.