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Updated: Jun 28, 2025

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将纤维细胞重编程为人类iBlastoids

Jia Ping Tan^1,2,3, Xiaodong Liu^4,5,6,7, Jose M Polo^8,9,10,11,12

¹Department of Anatomy and Developmental Biology, Monash University, Clayton, Victoria, Australia.

Nature protocols

|April 17, 2024

概括

研究人员开发了诱导的芽细胞体 (iBlastoids),一种体外人类芽细胞体模型,使用体质重编程. 与目前的方法相比,这种新模型为研究早期人类发展提供了一个更复杂的系统.

科学领域:

发展生物学发展生物学
干细胞生物学干细胞生物学
生殖医学生殖医学

背景情况:

目前用于早期人类胚胎发生的模型,包括捐赠的胚胎细胞和简化的干细胞培养,在捕捉发育复杂性方面存在局限性.
多能和热囊细胞干细胞具有价值,但缺乏早期胚胎发育的时空和分子动力学.

研究的目的:

描述一种用于产生诱导的布拉斯托因子 (iBlastoids) 的协议,这是人体胚胎囊的体外集成模型.
为解读人类胚胎发生提供一种替代性和更复杂的体外模型.

主要方法:

人体皮肤纤维细胞的体质重编程,以产生重编程中间体.
随后从这些中间体中生成iBlastoids,这个过程大约需要27天.
对iBlastoids的表征和功能测定进行讨论.

主要成果:

成功生成了iBlastoids,一个模仿人类胚胎囊的体外模型.
提供了详细的协议,需要大约一年的细胞培养和重编程测试经验.
iBlastoids为研究早期人类发展提供了一个替代工具.

结论:

诱导的Blastoids (iBlastoids) 提供了一个新的,更复杂的体外模型来研究早期人类胚胎发生.
该协议促进科学界对人类发展的探索.
在研究中,iBlastoids 作为传统胚胎囊模型的有价值的替代品.

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