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在酸性和氧化应激下,Mycobacterium intracellulare中显著的转录变化

Hyun-Eui Park¹, Kyu-Min Kim^1,2, Jeong-Ih Shin^1,2

¹Department of Microbiology, College of Medicine, Gyeongsang National University, Jinju, 52727, Republic of Korea.

|April 17, 2024

概括

细胞内菌菌的存活取决于应激反应. 氧化应激激活DNA修复基因,而酸性条件涉及和硫代谢,为非结核菌肺病提供潜在的治疗点.

科学领域:

微生物学微生物学
分子生物学分子生物学
基因组学就是基因组学.

背景情况:

包括Mycobacterium intracellulare在内的Mycobacterium avium复合体 (MAC) 在免疫受损和老年人中引起肺部疾病.
了解病原体适应机制是控制感染的关键.
在M. intracellulare中应激反应的途径仍然不完全理解.

研究的目的:

为了研究Mycobacterium intracellulare对酸性和氧化应激的转录反应.
在敌对条件下识别基因和通路,这些基因和通路对于M.细胞内生存至关重要.

主要方法:

对暴露于10mM过氧化 (氧化应激) 和pH4.5 (酸性应激) 的细胞内M.的转录组分析.
不同基因表达分析 (FC ≥2.0,p <0.05).
不同表达基因的功能丰富分析.

主要成果:

氧化应激 (H2O2) 影响了80个基因,其中77个被上调,主要涉及DNA复制和修复通路 (例如,recA,dnaB).
酸性压力 (pH4.5) 影响了878个基因,其中339个是上调的,539个是下调的,突出了和硫代谢途径 (例如,narGHIJ,cysND).
特定的基因,如dnaB,dinG,urvB, uvrD2, recA, narGHIJ, nirBD, narU, narK3, cysND, cysC, cysH,ferredoxin 1 和 2,以及形式脱酶被确定为对抗压力的关键.

结论:

细胞内M.激活不同的转录途径以生存酸性和氧化应激.
基因复制和修复对于抗氧化压力至关重要.
和硫代谢对于酸性应激适应至关重要.
这些应激反应途径代表了M.细胞内感染的潜在治疗点.

关键词:

这种复杂的菌株是Mycobacterium avium.这是一种细胞内菌菌 (Mycobacterium intracellulare).菌根细菌 (Mycobacteria) 是一种细菌.的新陈代谢的新陈代谢没有结核的真菌菌.在RNA-seqqq.抗压力抗压力抗压力硫的新陈代谢硫的新陈代谢

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