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长寿蛋白质和DNA作为组织相关疾病的候选预测生物标志物
Xiaosong Liu1,2, Bozidar Novak3, Christian Namendorf4
1Interdisciplinary Research Center on Biology and Chemistry, Shanghai Institute of Organic Chemistry, Chinese Academy of Sciences, 100 Haike Road, Shanghai 201210, China.
iScience
|April 18, 2024
概括
这项研究绘制了小鼠的长寿蛋白 (LLPs),揭示了组织特异性模式. 这些蛋白质随着时间的推移而积累损伤,可以作为衰老和相关疾病的生物标志物.
科学领域:
- 生物化学 生物化学
- 蛋白质组学是指蛋白质组学
- 衰老研究研究 衰老研究
背景情况:
- 蛋白质循环对于维持细胞健康 (蛋白质稳定) 是至关重要的.
- 长寿命蛋白 (LLPs) 随着时间的推移而积累损伤,冒着功能衰退的风险.
- 了解LLP动态对于衰老和疾病研究至关重要.
研究的目的:
- 在各种小鼠组织和血中识别和绘制长寿命蛋白 (LLPs).
- 研究LLP积累,DNA更新和与年龄相关的病理之间的关系.
- 探索LLP作为衰老和相关疾病的生物标志物的潜力.
主要方法:
- 在小鼠中从出生到成年 (第89天) 在体内使用稳定同位素标记.
- 在十个组织和血样本上进行了定量蛋白质组学分析.
- 量化脱氧核化物用于评估细胞DNA更新率.
主要成果:
- 确定了2113种长寿命蛋白 (LLPs),具有广泛分布和组织特异分布.
- 在血中检测到大量的LLP,这表明与心血管疾病的联系.
- 发现大脑LLP和DNA更新率之间的相关性,涉及神经退行性疾病途径.
- 生成了老鼠LLP的全面,组织特定的地图.
结论:
- 组织特定的LLP地图突出显示了由于更新率低而易受损伤的蛋白质.
- 血衍生LLP显示为衰老和与年龄有关的心血管疾病的生物标志物具有前途.
- 大脑LLPs可能与神经退行性疾病有关,需要进一步调查.
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