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蒂迪亚苏龙与环化物结合,调节棉花中的激素通路和ROS系统,在低温下增加脱叶
Hongmei Shu1, Shangwen Sun1, Xiaojing Wang1
1Institute of Industrial Crops, Jiangsu Academy of Agricultural Sciences/Key Laboratory of Cotton and Rapeseed, Ministry of Agriculture and Rural Affairs, Nanjing, China.
Frontiers in plant science
|April 18, 2024
概括
环化物 (CYC) 与滴亚祖龙 (TDZ) 结合,通过调节植物激素和活性氧物种,在低温下增强棉花脱叶. 这种组合可以提高脱叶率,当温度仅对TDZ而言不足于最佳时.
科学领域:
- 农业科学 农业科学
- 植物生理学 植物生理学
- 分子生物学分子生物学
背景情况:
- 低温降低了蒂迪亚祖龙 (TDZ) 对棉花脱叶的有效性.
- 已知环化物 (CYC) 可以在寒冷条件下提高TDZ的脱叶效率,但根本机制尚不清楚.
研究的目的:
- 研究CYC在低温 (15°C) 中增强TDZ诱导的棉花叶脱落的生理和分子机制.
主要方法:
- 在受控的低温条件下对棉花植物应用TDZ和CYC.
- 叶子脱落和脱叶率的生理测量.
- 分离区 (AZs) 的转录组分析 (RNA-Seq),以确定差异表达基因 (DEGs).
- 基因本体学 (GO) 和基因和基因组的京都百科全书 (KEGG) 路径分析.
- 植物激素水平的量化 (印醇-3-酸,雅斯蒙酸,1-氨基cyclopropane-1-carboxylic 酸) 和活性氧物种 (ROS) 含量.
主要成果:
- 与15°C单独使用TDZ相比,TDZ+CYC显著加快了叶子脱落和增加了脱叶率.
- 转录组分析显示,TDZ+CYC组中更多的DEG,主要与植物激素和ROS通路有关.
- TDZ + CYC改变了激素平衡,通过对辅酶反应基因进行下调和对乙烯和酸 (JA) 反应基因进行上调.
- 这种治疗降低了英多尔-3-酸 (IAA) 含量,同时增加了JA和1-aminocyclopropane-1-carboxylic acid (ACC) 水平.
- 在AZ中,TDZ + CYC上调了呼吸口爆氧化酶同类物 (RBOH) 和过氧化 (H2O2) 含量增加.
结论:
- 在低温下,CYC提高了棉花中TDZ脱叶的效率.
- 这种增强是由辅素,乙烯和JA信号通路的调节介导的.
- 由上调的RBOH基因驱动的ROS产量的增加,特别是H2O2,也导致了加速的脱叶.
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