血清N-糖性分析确定了评估冠状动脉狭窄症严重程度的候选生物标志物面板
Linlin Wu1, Haoqi Liu2, Xuewen Xu1
1Department of Clinical Laboratory Medicine Center, Yueyang Hospital of Integrated Traditional Chinese and Western Medicine, Shanghai University of Traditional Chinese Medicine, China.
血清N-甘氨酸显示出作为诊断冠状动脉疾病 (CAD) 狭窄程度的非侵入性工具的潜力. 这对于许多患者来说,可以提供侵入性冠状动脉血管学 (ICA) 的替代方案.
科学领域:
- 生物化学 生物化学
- 心血管医学 心血管医学
- 生物标志物发现发现
背景情况:
- 冠状动脉疾病 (CAD) 严重程度的进展增加了死亡风险.
- 侵入性冠状动脉血管学 (ICA) 是狭窄症评估的标准,但有其局限性.
- 血清N-甘氨酸样本与心血管疾病有关,但它们在CAD狭窄症中的诊断作用尚不清楚.
研究的目的:
- 研究血清N-甘氨酸在诊断CAD狭窄症严重程度方面的潜力.
- 探索N-glycans作为ICA的非侵入性替代品.
主要方法:
- 使用MALDI-TOF质谱分析了来自132名CAD患者和27名健康对照者的血清N-葡萄糖.
- 将患者分为四个狭窄症严重程度组 (没有/轻度/中度/严重).
- 利用统计分析 (LASSO,逻辑回归) 来开发诊断模型.
主要成果:
- 量化了27个N-甘氨酸和衍生了47个甘氨酸特征,确定了狭窄群体中的18种变异.
- 开发了五种诊断模型,用于识别狭窄度.
- 证明改变的血清N-糖化与狭窄的严重程度相关.
结论:
- 血清N-甘氨酸的变化可以作为有价值的生物标志物,用于识别CAD狭窄程度.
- 这种方法可以为ICA提供一种非侵入性诊断替代方案.
- 进一步的多中心,大型队列验证是必要的,以确认N-甘氨酸面板的诊断潜力.
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