通过使用寿命工程来设计,制造和测试复合性 Bacillus subtilis 底盘细胞,用于强大的生物工艺
Kexin Ren1,2, Qiang Wang1,2, Jianghua Chen1
1Key Laboratory of Industrial Biotechnology of the Ministry of Education, School of Biotechnology, Jiangnan University, Wuxi, Jiangsu, 214122, China.
Synthetic and systems biotechnology
|April 18, 2024
概括
这项研究使用寿命工程设计了Bacillus subtilis细胞工厂,以提高化学生产. 改造后的菌株在l-glutaminase和α-arbutin方面取得了创纪录的产量,提高了工业的可持续性.
科学领域:
- 生物技术和合成生物学
- 微生物工程是微生物的工程.
- 工业微生物学 工业微生物学
背景情况:
- 微生物细胞工厂从可再生资源提供可持续的化学生产.
- 细菌细菌是一种安全的工业宿主,但发酵限制仍然存在.
- 传统的代谢工程策略面临着诸如细胞自解和基质毒性等挑战.
研究的目的:
- 通过寿命工程开发强大的Bacillus subtilis底盘细胞.
- 克服微生物发酵的局限性,以提高工业化工生产.
- 为了提高细胞对有毒基质的耐受性和质量转移效率.
主要方法:
- 适用于Bacillus subtilis底盘细胞的寿命工程策略.
- 补充传统的代谢修饰方法.
- 工程用于缓解细胞自解和增强基质耐受性.
- 优化,以提高生物反应器中的质量转移效率.
主要成果:
- 修改后的底盘细胞有效地产生了高水平的l-胺酶和α-arbutin.
- 达到了2817.4±21.7U/mL的l-氨酶活性,增加1.98倍.
- 达到了134.7g/L的α-arbutin产量,增加了1.34倍,具有氨酸耐受性.
- 报道了两种产品迄今为止的最高产量.
结论:
- 寿命工程为强大的微生物细胞工厂设计提供了一种新的策略.
- 这种方法可以减轻广泛的代谢变化的不良影响.
- 提高细胞利用效率,为微生物细胞工厂的发展提供新的途径.
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