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通过使用长读序列测序,组装Hemicentrotus pulcherrimus的连续高分辨率基因组序列草案

Tetsushi Komoto¹, Kazuho Ikeo², Shunsuke Yaguchi³

¹Graduate School of Integrated Sciences for Life, Hiroshima University, Higashi-Hiroshima, Japan.

Development, growth & differentiation

|April 18, 2024

概括

使用长读测序的新海基因组组合为研究发育和基因调节提供了更高的分辨率. 这种改进的Hemicentrotus pulcherrimus基因组为生物研究提供了宝贵的资源.

科学领域:

基因组学就是基因组学.
发展生物学发展生物学
海洋生物学海洋生物学

背景情况:

在东亚,Hemicentrotus pulcherrimus是研究早期发育的关键模型生物.
以前的基因组组合草案在连续性和分辨率方面存在局限性.

研究的目的:

更新和改进Hemicentrotus pulcherrimus的基因组组合草案.
为开发,基因调节和核动力学研究提供更高分辨率的基因组资源.

主要方法:

利用牛津纳米孔长读测序技术进行基因组组装.
分析了连续性,N50,BUSCO完整性和转录组模型映射比率 (TMMR).

主要成果:

产生了一个600Mb的基因组组合,有2,163个contigs和N50的516 kb.
获得了96.5%的BUSCO完整度得分和77.8%的TMMR,明显改善了之前的组装.
鉴定了36,055个基因模型,双重重复序列的基因基因基因位,和Ars绝缘体的同源序列.

结论:

更新的Hemicentrotus pulcherrimus基因组组合提供了增强的连续性和分辨率.
这种改进的资源有助于在发育生物学,基因调节和核内结构动力学方面进行先进的全基因组研究.

关键词:

在ArsInsCC中使用.在 cis 调节序列中.长读序列编排长读序列编排重复序列重复序列的时间.海的基因组海洋

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Genome Annotation and Assembly

Genome Annotation and Assembly

The genome refers to all of the genetic material in an organism. It can range from a few million base pairs in microbial cells to several billion base pairs in many eukaryotic organisms. Genome assembly refers to the process of taking the DNA sequencing data and putting it all back together in a correct order to create a close representation of the original genome. This is followed by the identification of functional elements on the newly assembled genome, a process called genome annotation.