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在胚胎中早期索米特细分的时空动态

Ana Cristina Maia-Fernandes^1,2, Ana Martins-Jesus¹, Nísia Borralho-Martins^1,2

¹ABC-RI, Algarve Biomedical Center Research Institute, Faro, Portugal.

|April 18, 2024

概括

胚胎时钟 (EC) 控制脊椎动物的身体细分. 这项研究揭示了由毛的表达标记的EC振荡,从发育的最开始就与形形成相结合.

科学领域:

发展生物学发展生物学
遗传学遗传学是一个
时间生物学时间生物学

背景情况:

脊椎动物身体在前后轴上的细分是一个基本的发育过程.
由基因表达振荡 (例如,毛的1) 标志着体质生成胚胎时钟 (EC),调节体质形成速度.
之前的研究描述了后期索米特生成阶段 (索米特15-52) 的EC振荡,但早期动态仍然不清楚.

研究的目的:

研究胚胎时钟 (EC) 的周期性和表达动态,在胚胎早期的体质生成过程中.
为了确定正面大部分索米特形成的精确时间及其与EC振荡的关系.
从发展的开始,建立EC振荡和索米特细分之间的合.

主要方法:

利用高分辨率 (3分钟间隔) 的时间延迟成像,从原始的子胚胎到体质生成阶段.
开发了一种算法,可以自动测量somite长度,并从成像数据中推断形成时间.
评估了使用半胚胎探索物和活体成像外卵培养物的毛发表达力学.

主要成果:

尾索米特 (高达5个索米特) 形成的速度更快 (约. 75分钟间隔) 比随后的索米特 (大约. 90分钟的时间间隔).
胚胎时钟 (EC) 毛的表达表现出了在体质生成之前的动态行为,并在第一个体质形成时表现出明显的振荡.
实时成像证实,毛的表达模式与每一对新的索米特重复,证明了自发病以来的合.

结论:

在早期发育过程中,索米特形成速度有所变化,最初的索米特形成速度更快.
胚胎时钟 (EC) 从体质生成的最早阶段开始就活跃和振荡.
索米特细分从脊椎动物发育的最开始就与EC振荡密切相关.

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