用于实验室使用的Trypanosoma cruzi无活化技术的验证
Lorna M MacLean1, Mark Ariyanayagam2, Lalitha Sastry1
1Drug Discovery Unit, Wellcome Centre for Anti-Infectives Research, School of Life Sciences, University of Dundee, Dundee, United Kingdom.
PloS one
|April 18, 2024
概括
三种经过验证的方法有效地使Trypanosoma cruzi (T. cruzi) 不活化,这是导致查加斯病的寄生虫.
科学领域:
- 寄生虫学的寄生虫学
- 传染性疾病 传染性疾病
- 生物安全是生物安全.
背景情况:
- 草虫 (Trypanosoma cruzi) 导致查加斯病,在拉丁美洲是一个重大的健康问题.
- 目前对查加斯病的治疗方法有限,需要开发新的治疗策略.
- T. cruzi是一种危险组3的生物体,在实验室环境中需要严格的安全协议 (CL3).
研究的目的:
- 评估和验证三种不同的方法来禁用Trypanosoma cruzi.
- 在实验室环境中建立安全有效的处理和处置T. cruzi的程序.
- 为了确定可靠的无活化技术,适用于危险组2-3的kinetoplastid寄生虫.
主要方法:
- 在T. cruzi.上评估了三次冷解周期的无活化效果.
- 使用Virkon消毒剂在不同度和体积下进行评估的化学失活.
- 在Whatman FTA卡 (A,B,C,Elute) 和Mitra微量采样装置上测试了空气干燥失活.
主要成果:
- 三次冷解周期成功地使所有测试的T. cruzi生命阶段和细胞颗粒失活.
- 维尔康治疗 (≥1%度,十倍过量) 在血液和细胞培养中有效地使T. cruzi无活化.
- 空气干燥使T. cruzi在FTA A,B,Elute卡 (≥30分钟) 和Mitra设备 (2小时) 上失活,但没有FTA C卡.
结论:
- 冷解周期,Virkon处理和特定FTA卡/Mitra设备上的空气干燥是经过验证的T. cruzi无活化方法.
- 这些方法确保了T. cruzi废物的安全处置和CL3设施内的安全处理.
- 这些发现支持对相关的kinetoplastid寄生虫的这些验证的失活技术的应用.
相关概念视频
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