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伪狂犬病病毒操纵线粒体三聚乙烯-tRNA合成酶2进行病毒复制

Xiu-Qing Li¹, Meng-Pan Cai¹, Ming-Yang Wang¹

¹College of Veterinary Medicine, Henan Agricultural University, Zhengzhou 450046, China; Key Laboratory of Animal Biochemistry and Nutrition, Ministry of Agriculture and Rural Affairs, Zhengzhou 450046, China; Key Laboratory of Veterinary Biotechnology of Henan Province, Henan Agricultural University, Zhengzhou 450046, China.

Virologica Sinica

|April 18, 2024

概括

伪狂热病毒 (PRV) 感染增加了三甲基-tRNA合成酶2 (WARS2) 的水平. 通过管理蛋白质和脂质合成,WARS2促进了PRV感染,提供了新的治疗点.

科学领域:

病毒学病毒学
分子生物学分子生物学
生物化学生化学

背景情况:

伪狂热病毒 (PRV) 导致奥耶斯基病,导致全球经济遭受重大损失.
甲基-tRNA合成酶2 (WARS2) 是一种线粒体酶,对蛋白质合成至关重要,并且被广泛表达.

研究的目的:

研究WARS2在PRV感染中的作用.
为了阐明PRV感染期间WARS2的调节机制.
确定WARS2如何影响病毒复制和宿主细胞代谢.

主要方法:

定量实时PCR和西部抹迹测量WARS2mRNA和蛋白质水平.
通过RNA干扰 (RNAi) 抑制WARS2的表达.
细胞培养和动物模型用于PRV感染研究.
信号通路的分析,包括cGAS/STING/TBK1/IRF3和I型干扰素信号.
评估蛋白质和脂质合成速度.

主要成果:

在体外和体内,PRV感染显著上调WARS2mRNA和蛋白质水平.
抑制WARS2降低了PRV感染率,而增强增加了它们.
通过PRV激活WARS2是由cGAS/STING/TBK1/IRF3通路和干扰素α受体-1介导的.
降低WARS2水平损害了PRV诱导的蛋白质和脂质合成.

结论:

在促进PRV感染方面,WARS2起着至关重要的作用.
通过调节宿主细胞蛋白质和脂质合成,WARS2支持PRV复制.
准WARS2为开发针对PRV的新型抗病毒疗法提供了潜在的战略.

关键词:

这是天生的免疫力.脂质合成脂质合成蛋白质合成蛋白质合成伪狂犬病病毒病毒.战争2 战争2

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